Dow Agrosciences LLC

Pioneer Hi-bred International, Inc.

E.i. Dupont De Nemours and Company

Sangamo Therapeutics, Inc.

Sangamo Biosciences, Inc.

Corteva Agriscience LLC

Kumar, Sandeep

Alabed, Diaa

Gupta, Manju

Manju Gupta

Kenneth Edwin Narva

James Wayne Bing

Robert F Cressman, Jr

David Hondred

Daniel Moellenbeck

Mary E Hartnett Locke

Craig D Sanders

Sandeep Kumar

Todd L Krone

Jimei Wang

Abigail K Luckring

Gan-Yuan Zhong

Salim M Hakimi

Paul D Olson

Sandra E Meyer

Jian Zhang

Diaa Alabed

Jeffrey C Miller

Michael G Koziel

Laura Ann Tagliani

Terry EuClaire Meyer

Guy A Cardineau

Stephen N Novak

Dmitry Y Guschin

Joseph W Keaschall

David S Stucker

Eric Barbour

Steven Jeffrey Stelman

Wilas Nirunsuksiri

Marjorie L Rudert

Aihua Shao

William M Van Zante

Terry R Wright

Sara Bennett

Fyodor Urnov

German Carlos Spangenberg

Steven Robert Webb

Matthew J Henry

John White Forster

Steven W Ritchie

Noel Cogan

Matthew Hayden

Tim Sawbridge

Susan M Jayne

Doug A Smith

Jeffrey Beringer

Navin Elango

Karthik Narayna Muthuraman

Huixia Wu

Joseph F Petolino

Lakshmi Sastry-Dent

W Michael Ainley

Russell DeKelver

Paul Bundock

Vipula Shukla

Wei Chen

Asha Mehra Palta

Nagesh Sardesai

Sunita Gopalan

Michiel Jan De Both

William M Ainley

Andrew F Worden

Steven Arnold Thompson

Mandy Lynne Sullivan-Gilbert

Xueyi Hu

Stephen W Ritchie

German Spangenberg

William Michael Ainley

John Forster

Asha M Palta

Robert F Cressman

Donald J Merlo

Jyoti R Rout

Sean Michael Russell

Steven Lee Evans

Liu Shen

John Mason

Zoe Christina Ehlert

Sivarama Reddy Chennareddy

Lasantha Chandana Ubayasena

Chandra Shekara A Channabasavaradhya

W Mike Ainley

Susan Jayne

Shavell Gorman

Russell Dekelver

Hartnett Locke Mary E

Karthik N Muthuraman

Steven J Stelman

Disclosed herein are methods and compositions for targeted modification of one or more endogenous plant malate dehydrogenase genes.

Targeted modification of malate dehydrogenase

The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DAS-59122-7 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.

Corn event DAS-59122-7 and methods for detection thereof

This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing aGRMZM2G144030 promoter. Some embodiments relate to aGRMZM2G144030 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to aGRMZM2G144030 3'UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.

Plant promoter and 3'UTR for transgene expression

A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

FAD3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks

An Engineered Transgene Integration Platform (ETIP) is described that can be inserted randomly or at targeted locations in plant genomes to facilitate rapid selection and detection of a GOI that is perfectly targeted (both the 5' and 3&#x2032; ends) at the ETIP genomic location. One element in the subject disclosure is the introduction of specific double stranded breaks within the ETIP. In some embodiments, an ETIP is described using zinc finger nuclease binding sites, but may utilize other targeting technologies such as meganucleases, CRISPRs, TALs, or leucine zippers. Also described are compositions of, and methods for producing, transgenic plants wherein the donor or payload DNA expresses one or more products of an exogenous nucleic acid sequence (e.g. protein or RNA) that has been stably-integrated into an ETIP in a plant cell. In embodiments, the ETIP facilitates testing of gene candidates and plant expression vectors from ideation through Development phases.

Engineered transgene integration platform (ETIP) for gene targeting and trait stacking

A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

FAD2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks

This disclosure concerns compositions and methods for promoting transcription and translation of a nucleotide sequence in a plant or plant cell, employing a 3'UTR fromchlorophyll a/b binding protein gene. Some embodiments relate to a 3&#x2032; UTR from achlorophyll a/b binding protein gene that functions in plants to terminate transcription of operably linked nucleotide sequences.

Plant promoter and 3' UTR fromchlorophyl A/B binding protein gene

This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing aGRMZM2G047720 promoter. Some embodiments relate to aGRMZM2G047720 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to aGRMZM2G047720 3'UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.

The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes.

Engineered zinc finger proteins targeting 5-enolpyruvyl shikimate-3-phosphate synthase genes

This disclosure concerns compositions and methods for promoting transcription and translation of a nucleotide sequence in a plant or plant cell, employing a 3'UTR fromUbiquitin C9 gene. Some embodiments relate to a 3&#x2032; UTR from aUbiquitin C9 gene that functions in plants to terminate transcription of operably linked nucleotide sequences.

Plant promoter and 3' UTR for transgene expression

Provided are vector constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements, including the promoters, 5'-UTRs, and/or 3&#x2032;-UTRs, isolated from

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Provided are vector constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements, including the promoters, 5'-UTRs, introns, and/or 3&#x2032;-UTRs, isolated from

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Carmel, IN, United States of America

Manju Gupta