Butamax (tm) Advanced Biofuels LLC

E.i. Dupont De Nemours and Company

Butamax Advanced Biofuels LLC

Manjari Singh

Michael G Bramucci

Vasantha Nagarajan

Natalia Sedkova

Dennis Flint

Tina K Van Dyk

Edward S Miller, Jr

Robert A Larossa

Edward S Miller Jr

Increasing tolerance to butanol in yeast has been accomplished by increasing activity of the filamentous growth response. Yeast with increased expression of MSS11p, a transcriptional activator of the filamentous growth response pathway had increased tolerance to isobutanol. These yeast may be used for improved butanol production.

Yeast with increased butanol tolerance involving filamentous growth response

A method for the production of 1-butanol by fermentation using a microbial production host is disclosed. The method employs a reduction in temperature during the fermentation process that results in a more robust tolerance of the production host to the butanol product.

Method for the production of 1-butanol

A method for the production of 2-butanol by fermentation using a microbial production host is disclosed. The method employs a reduction in temperature during the fermentation process that results in a more robust tolerance of the production host to the butanol product.

Method for the production of 2-butanol

bacteria having enhanced tolerance to butanols have been isolated. The bacteria are useful for the fermentive production of butanol. New methods for the isolation of butanol tolerantare also provided.

Solvent tolerant microorganisms and methods of isolation

Growing community of inventors

West Chester, PA, United States of America

Manjari Singh