Council of Scientific and Industrial Research

Manish Kulshrestha

Sushil Kumar

Sunil Kumar Chattopadhyay

Ram Prakash Sharma

Suchitra Banerjee

Vijay Kumar Mehta

Shipra Agarwal

Koneni Venkata Sashidhara

Vinayak Tripathi

Arun Kumar Kukreja

The invention provides a process for the production of a biologically active phenolic compound (&plus;) catechin from tissue cultures, said process comprising the steps of (a) inoculating the of explants on a culture medium supplemented with combinations of auxins and cytokinins, (b) incubating the cultures under continuous light or dark conditions for 4-6 weeks for callus initiation followed by subculturing at 4-6 weeks intervals, (c) extracting the fresh pulverized calli with polar solvents at room temperature; (d) evaporating the solvent to obtain a residue; and (e) treating the residue with a chlorinated solvent and isolating (&plus;) catechin compound by filtration.

Process for the production of a biologically active phenolic compound(&plus;) catechin

This invention relates to a process for the production of a compound (&plus;) catechin penta acetate of formula I from tissue cultures which comprises : (a) inoculation of explants on different media compositions supplemented with combinations of auxins (1-5 mg/l) and cytokinins (0.1-1.0 mg/l); (b) incubation of the cultures under continuous light or dark conditions for 4-6 weeks for callus cultures followed by subculturing at 4-6 weeks intervals; (c) harvesting of the calli at different growth phases ranging from 12-36 months; (d) extraction of fresh pulverized calli with polar solvents at room temperature; (e) evaporating the solvent to give a residue; and partitioning of the residue between water and chlorinated solvents and evaporating the solvent to a semisolid mass; (f) subjecting the resultant mass to column chromatography over suitable adsorbent and (g) eluting with organic solvent/mixtures of organic solvents to get (&plus;) catechin penta acetate.

Process for the production of a compound (&plus;) catechin penta acetate useful as a precursor for the production of (&plus;) catechin

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Lucknow, India

Manish Kulshrestha