Harvard College

Leland Stanford Junior University

The Charles Stark Draper Laboratory, Inc.

Manish J Butte

George M Whitesides

Katherine Andrea Mirica

Scott T Phillips

Samuel W Thomas

Emanuel Carrilho

Hayat Sindi

Sarah J Vella

Yanyan Liu

Amy Wong

Andres W Martinez

Olav Solgaard

Jayakumar Rajadas

John Joseph LeBlanc

Michael D Dickey

Andreas W Martinez

Marc Amor Bruce

Jianwei Liu

Meital Reches

Antonio A Gellineau

Karthik Vijayraghavan

Andrew Y J Wang

Sarah Jane Vella

A method of testing for specificity and strength of an allergic reaction is performed by penetrating the skin of an individual with the microneedle array comprising a plurality of epitopes and determining the response of the skin to the allergens. The response can be measured with a thermal imaging device. Analysis, and optionally treatment, can be provided to the individual.

Methods of testing for allergen sensitivity

Embodiments of the invention provide lateral flow and flow-through bioassay devices based on patterned porous media, methods of making same, and methods of using same. Under one aspect, an assay device includes a porous, hydrophilic medium; a fluid impervious barrier comprising polymerized photoresist, the barrier substantially permeating the thickness of the porous, hydrophilic medium and defining a boundary of an assay region within the porous, hydrophilic medium; and an assay reagent in the assay region.

Lateral flow and flow-through bioassay devices based on patterned porous media, methods of making same, and methods of using same

A high-bandwidth AFM probe having a diffraction grating characterized by a diffraction characteristic that monotonically changes along the length of the diffraction grating is disclosed. AFM probes in accordance with the present invention are capable of high-sensitivity performance over a broad range of operating conditions, such as operating wavelength and measurement media. A method for estimating at least one physical property of a surface based on high-frequency signal components in the output signal from a high-bandwidth AFM probe is also disclosed. The method enables determination of tip-surface interaction forces based on the relationship between a first motion of the base of the AFM probe and a second motion of the tip of the AFM probe.

Interferometric atomic-force microscopy device and method

A microfluidic detection device is provided that includes a planar waveguide, or an ion-exchange planar waveguide, a microfluidic channel disposed on the planar waveguide, a light source, such as a laser, LED or incandescent light, directed through the planar waveguide, a labeled cell disposed in the microfluidic channel, where the labeled cell lies in an evanescent field extending from the planar waveguide, and a light detector disposed to receive light from the light source through the planar waveguide. The evanescent field interacts with the labeled cell, where the light through the planar waveguide is altered according to a presence of the labeled cell in the microfluidic channel.

Microfluidic waveguide detector

Techniques for atomic force microscope manipulation of living cells include functionalizing a nanoscale tip of a microscale cantilever with a first ligand for a first receptor associated with a surface of a first type of cell. The method further comprises, controlling the cantilever to cause the first ligand on the nanoscale tip to contact the first receptor on a surface of a living cell of the first type in a particular temporal pattern to induce a target response by the living cell. Other techniques for controlling an atomic force microscope comprising a nanoscale tip include controlling the cantilever to cause the nanoscale tip to contact a living cardiomyocyte at a predetermined pressure. The cantilever is also controlled to turn off vertical deflection feedback after contacting the cardiomyocyte and collecting deflection data that indicates a time series of nanoscale vertical deflections of the microscale cantilever caused by the living cardiomyocyte.

Atomic force microscope manipulation of living cells

Hydrophilic threads as platforms for inexpensive, low volume, portable diagnostic systems, and methods of making the same are described. A diagnostic system includes a hydrophilic loading thread having an inlet zone at a proximal end; a testing zone at a distal end; and an intermediate zone located between the inlet zone and the testing zone, wherein the testing zone does not directly contact the inlet zone. In another aspect, a diagnostic system includes (i) a hydrophilic loading thread that includes an inlet zone at a proximal end and an intermediate zone at a distal end; and (ii) one or more additional hydrophilic threads that contact the intermediate zone of the loading thread. A method of detecting the presence or absence of an analyte in a fluid sample includes applying the sample to an inlet zone of a diagnostic system that includes a hydrophilic loading thread with an inlet zone at a proximal end; an intermediate zone; and a testing zone at a distal end; wherein the testing zone does not directly contact the inlet zone.

Cotton thread as a low-cost multi-assay diagnostic platform

Growing community of inventors

Boston, MA, United States of America

Manish J Butte