Nikon Corporation

The University of Tokyo

National Institute of Advanced Industrial Science and Technology

Japan Science and Technology Agency

Janusys Co., Ltd.

Manish Biyani

Takanori Ichiki

Hirofumi Shiono

Ryo Kobayashi

Shingo Ueno

Naoto Nemoto

Naota Nemoto

The present invention relates to a nucleic acid linker for producing a complex of mRNA, and a protein or a peptide which is encoded by the mRNA, the linker comprising: a spacer portion at the 5'-terminal; a polynucleotide portion hybridizable with at least a part of a sequence of the mRNA; and an arm portion which has a connection portion for the protein or the peptide at the 3&#x2032;-terminal, in which the spacer portion, the polynucleotide portion, and the arm portion form a single strand, and in which the polynucleotide portion contains a photoreactive base derivative.

Nucleic acid linker

The present invention relates to a protein or peptide printing method, comprising (a) a step for preparing nucleic acids and a cell-free protein synthesis system in an engraved plate composed of microscopic grooves having a specific opening shape, (b) a step for superimposing a substrate on the engraved plate so as to contact a protein or peptide to be synthesized in the microscopic grooves, and (c) a step for synthesizing the protein or peptide from the nucleic acids using the cell-free protein synthesis system in the microscopic grooves, and immobilizing the protein or peptide on the substrate along the specific opening shapes of the microscopic grooves.

Protein or peptide printing method, protein array or peptide array, and functional protein or functional peptide identification method

A method for producing a chip on which biomolecules are immobilized in an aligned state, comprises (a) producing a substrateon which a plurality of biomoleculesof a single type are immobilized in an aligned state, (b) adding reaction reagents for synthesizing biomoleculesto microreactors on a microreactor chip comprising the microreactors at positions overlapping with the sequence positions of the biomoleculesimmobilized on the substrateproduced in step (a), (c) closely attaching the microreactor chip to the substrateso that the reaction reagents for synthesizing the biomoleculesare allowed to come into contact with the biomolecules, so as to synthesize the biomoleculesin the microreactors, and (d) superposing the microreactor chip on a substrateafter completion of step (c) so as to bind the biomoleculesonto the substrate; and a chip produced thereby.

Biomolecule assay chip

The present invention provides a linker preferably used when constructing an mRNA/cDNA-puromycin-protein conjugate used in an in vitro virus method, and an mRNA/cDNA-puromycin-protein conjugate constructed using that linker. More specifically, the present invention provides a linker for ligating mRNA and puromycin or a puromycin-like compound to construct an mRNA/cDNA-puromycin-protein conjugate, the linker comprising a single-stranded RNA as a main backbone, and having, in this main backbone, a solid phase binding site for binding an mRNA-puromycin-protein conjugate to a solid phase site, and a pair of cleavage sites provided at locations surrounding the solid phase binding site; an mRNA-puromycin-protein conjugate constructed using this linker; an mRNA bead or an mRNA chip comprising this conjugate; a protein chip produced from this mRNA chip; and a diagnostic kit using the mRNA bead or the mRNA chip.

Linker for constructing mRNA-puromycin-protein conjugate

Growing community of inventors

Tokyo, Japan

Manish Biyani