Deutsches Krebsforschungszentrum Stiftung Des Offentlichen Rechts

Deutsches Krebsforschungszentrum

Forschungszentrum Juelich Gmbh

Ruprecht-karls-universitat Heidelberg

Ruprecht-karls-universitaet Heidelberg

Deutsches Krebsforschungszentrum Stiftung Des Oeffentlichen Rechts

Forschungszentrum Julich Gmbh

Manfred Wiessler

Peter Lorenz

Heinz Fleischhacker

Walter Mier

Michael Trendelenburg

Stefan Raddatz

Eberhard Spiess

Christian Kliem

Andres Jäschke

Dirk Mayer

Andreas Offenhaeusser

Jörg Hoheisel

Karsten Richter

Ansgar Haking

Konrad Beyreuther

Helmut Tr&ouml;ster

Sandra Gilles

Hans-Christian Kliem

Marek Los

Klaus Michael Debatin

Markus Gschrey

Willi Von Der Lieth

Simone Fulda

Dagmar Sch&uuml;tte

Michael Dickes

Birgit Schmauser

Sabine Bub

Helmut Troester

Bernd Sauerbrei

Anette Jacob

Eduard Müller

Juliane Schoch

Ranjita Ghosh-Moulick

Ayan Samanta

Juliane Becker

Marc Dauber

Andreas Offenhäuser

In summary, the present invention concerns a method for multiple orthogonal labelling of oligonucleotides, preferably RNA or DNA, by simultaneously performing the inverse Diels-Alder reaction (DAinv) and the copper-catalyzed click reaction (CuAAC), wherein the method is employed in a single step by just adding the different reaction components together and incubating the aqueous reaction mixture preferably for one hour at room temperature. In detail, the reaction components are one or more N-modified labels, a copper compound, a stabilizing ligand, a reducing agent and one or more electron-deficient label-modified dienes that are added together with an at least double-modified oligonucleotide having one more nucleotides containing one or more N3-reactive groups and one or more electron-rich dienophiles, wherein a terminal alkyne moiety is preferably used as N3-reactive group(s) and a frans-cyclooctene moiety or norbornene is preferably used as electron-rich dienophile(s), more preferably frans-cyclooctene. Therefore, the present invention provides a one-pot method for post-synthetic multiple orthogonal labeling of oligonucleotides, which allows the site-specific introduction of more than one label, preferably of at least two labels into oligonucleotides after solid-phase synthesis, wherein the DAinv takes place on the dienophile modification only and the CuAAC selectively takes place on the N3-reactive group modification.

Multiple orthogonal labelling of oligonucleotides

The present invention concerns a method for applying a first metal onto a second metal, an isolator or semiconductor substrate by a Diels-Alder reaction, in particular a Diels-Alder reaction with inverse electron demand. The present invention further concerns the binding units L 1960 and F 160.

Method for applying a first metal onto a second metal, an isolator or semiconductor substrate, and the respective binding units

The present invention concerns a method and a kit for the post-synthetic modification of nucleic acids via an inverse Diels-Alder reaction.

Post-synthetic modification of nucleic acids by inverse diels-alder reaction

The present invention relates to a process for linking two molecules by means of a Diels Alder reaction with inverse electron requirement (DARinv), comprising the following steps: reaction of a (a) triazine or tetrazine with one or more electron-attracting substituents on the ring as a diene component, the electron-attracting substituents being selected from:

Process for the covalent coupling of two molecules by means of a diels-alder reaction with inverse electron requirement

The present invention relates to a method by which saccharide compounds can be prepared in a very easy way. This method comprises the steps of:

Method for producing water-soluble saccharide conjugates and saccharide mimetics by Diels-Alder reaction

The invention relates to plasmids derived from pBluescript KS(&plus;), which contains more than 1, preferably 2, 7, 14, 21 and 27, respective SK primer elements, and their use in analytical electron microscopy.

Molecular-biological marker for analytical electron microscopy

Compounds containing boron for energy-filtering transmission electron microscopy and for boron neutron-capture therapy

This invention relates to a conjugate, comprising a saccharide and one or more therapeutic or diagnostic agents, as well as the use of the conjugate. The conjugate of the present invention may be used for diagnosing cancer or viral diseases. The conjugate of the present invention may also be used to prevent and/or treat neurological diseases.

Saccharide conjugates

The invention relates to dendrimers comprising an initiator core with at least two functional groups and at least two saccharides, a process for preparing sch dendrimers as well as uses thereof.

Dendrimers based on saccharides

The present invention is, generally, directed to the use of betulinic acid and derivatives thereof for the treatment of neuroectodermal tumors. The present invention is based on the discovery that betulinic acid and its derivatives are potent anti-neuroectodermal agents. As disclosed herein, betulinic acid and its derivatives are useful for the treatment of neuroectodermal tumors, including, due to its distinct mechanism of action, neuroectodermal tumors that are resistant to conventional chemotherapeutical agents. In addition to the new use of known compounds, the invention discloses novel compounds and pharmaceutical compositions for the treatment of neuroectodermal tumors.

Betulinic acid and derivatives thereof useful for the treatment of neuroectodermal tumor

The invention concerns metallocene-phosphoramidite conjugates comprising one or a plurality of metallocenes and one or a plurality of phosphoramidites. The invention further concerns a process for preparing the metallocene-phosphoramidite conjugates and their use.

Metallocene-phosphoramidite conjugates, process for their preparation and their use

Tumor inhibiting saccharide conjugates

Growing community of inventors

Frankenthal, Germany

Manfred Wiessler