Roche Diagnostics Operations, Inc.

Acea Biosciences, Inc.

Ciba-geigy Corporation

Roche Molecular Systems, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Manfred Watzele

Bernd Buchberger

Xiaobo Wang

Wei Li

Nan Li

Tianxing Wang

Alexander Seiler

Donald Thomas

Claudia Kirr

Dieter Heindl

Herbert Von Der Eltz

Frank Bergmann

Yama A Abassi

Horst Menzler

Hans Schels

Ning Ke

Erhard Fernholz

Thomas Metzler

Ulrike Fischer

Wolfgang Mutter

Horst Donner

Nina Lassonczyk

Albert Roeder

Stephanie Wessner

Svetoslav X Iwanow

Eric G Berger

Marcus Schmid

Dorothee Ambrosius

Cordula Nemetz

Thomas Hoffmann

Hans-Juergen Rode

Thomas Nikolaus

Adrian Papas

Cordula Jany

Hans-Jürgen Rode

The present invention deals with reagents and compositions capable of effectively inhibiting peroxidase activity. According to the invention, peroxidase enzymatic activity is blocked with an acidic aqueous solution of a protein denaturing agent. Preferred protein denaturing agents are detergents and chaotropic substances.

Inhibition of peroxidase enzymatic activity

An insert device and co-culture device assembly incorporating the insert device, which includes an insert chamber with two fluid impermeable side walls that extend from a microporous bottom to an open top to form an inner cavity. The first side wall is shaped to form a convex arc that follows between about half of a circumference of the well and less than an entire circumference of the well. The second side wall joins ends of the first side wall and protrudes inward towards a center of the convex arc. A flange extends outward from or beneath the top and is notched to form a gap adjacent to the second side wall, which forms an access port allowing access to the lower chamber with a micropipette tip when the insert device is inserted into a well of a single or multi-well plate.

Co-culture device assembly

The present invention deals with reagents and compositions capable of effectively inhibiting peroxidase activity. According to the invention, peroxidase enzymatic activity is blocked with an acidic aqueous solution of a protein denaturing agent. preferred protein denaturing agents are detergents and chaotropic substances.

Set of inserts for co-culture of cells in microtiter plates

The invention provides the use of tetraethylene glycol dimethyl ether for adsorbing nucleic acids to solid phases such as those with silica surfaces. To this end, the invention also provides compositions comprising TDE. Methods are disclosed and claimed to purify nucleic acids from samples, as well as kits useful for performing these methods. Particularly, the invention encompasses methods for the purification of nucleic acids with low molecular weight. The nucleic acids purified by a method of the invention are suited for assays aiming at the detection of a target nucleic acid.

Use of TDE for isolation of nucleic acids

Disclosed are methods and kits for measuring enzyme activity in which the turbidity of a sample caused by cell components is minimized by particular additives. The methods include the steps of providing a cell suspension or a cell lysate containing cell components, adding a chromogenic substrate at the start of the enzyme reaction, adding a first additional substance to stop the enzyme reaction, and making a colorimetric measurement, wherein a second additional substance which reduces the light scattering and/or absorption caused by the cells or the cell components is added during or after termination of the enzyme reaction. In particular, a method is disclosed wherein the enzyme is lactate dehydrogenase (LDH) and the first additional substance to stop the reaction is HCl.

Reducing sample turbidity in measurement of enzymatic activity in cell lysates

The invention is a multichamber microdialysis device with a multitude of sample chambers in close side by side arrangement (similar to a microtiter plate) for receiving liquid samples, and at least one dialysate chamber for receiving a dialysate liquid. The sample chambers are surrounded by circumferential sidewalls and are in liquid exchange contact to a neighboring dialysis chamber via an exchange opening covered by a semipermeable membrane. The semipermeable membrane is fixed liquid-tight to the walls of the sample chamber, so that a diffusion exchange between the sample chamber and the correspondingly neighboring dialysate chamber is only possible via the membrane. Consequently, only molecules with a molecular weight below the molecular cut-off of the semipermeable membrane can diffuse from the sample chamber to the dialysate chamber or from the dialysate chamber to the sample chamber.

Multichamber microdialysis device

The present invention concerns a method for improving the stability of linear short DNA towards exonucleases in cell-free in vitro transcription/translation systems using lysates containing exonucleases or in cellular systems containing exonucleases, wherein the stability of the linear short DNA is improved by adding unspecific linear DNA.

Method for improving the stability of linear DNA in cell-free in vitro transcription/translation systems

The invention concerns compounds which have a chromophore and a group capable of binding to streptavidin or/and avidin and which are suitable for binding to molecules containing an aldehyde, ketone, hemiacetal or/and hemiketal group. In addition the invention concerns conjugates formed from these compounds as well as a method for the detection or isolation of carbohydrates or glycan receptors by means of such conjugates.

Reagent for the detection and isolation of carbohydrates or glycan receptors

Proteins having glycosyltransferase activity

Growing community of inventors

Weilheim, Germany

Manfred Watzele