Macherey, Nagel Gmbh & Co.

Manfred Sieber

Robert Zeidler

The invention concerns a method for purifying biological macromolecules, e.g. nucleic acids or proteins, from starting materials, in which the macromolecules, contained in a biological solution, are purified on a chromatographic material, and in which endotoxins are partially or completely removed with the aid of a nonionic surfactant, which is characterized in that the surfactant and the biological solution are brought into contact immediately before or during the purification of the macromolecules on the chromatographic material.

Method for purifying biological marcromolecules, and chromatographic column for performing said method

Disclosed is an apparatus for isolating nucleic acids having a separation column which has a top inlet and a bottom outlet in which a separation device is arranged, and having a collection vessel for collecting liquid emerging from the outlet. The ratio between the internal volume of the separation column above the separation device and the surface area of the separation device is at least 10 ml/cm,.

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Nideggen, Germany

Manfred Sieber