Baxalta Gmbh

Baxter Healthcare Sa

Baxter International Inc.

Baxter Innovations Gmbh

Immuno Aktiengesellschaft

Takeda Pharmaceutical Company Limited

Baxter Aktiengesellschaft

Pharmacia Biotech Ab

Nanotherapeutics, Inc.

Vogelbusch Gesellschaft M.b.h.

Bluesky Immunotherapies Gmbh

Bexter Healthcare S.a.

Baxalta Innovations Gmbh

Manfred Reiter

Wolfgang Mundt

Leopold Grillberger

Friedrich Dorner

Artur Mitterer

Meinhard Hasslacher

Alexandra Spenger

Rana Grillberger

Daniel Fleischanderl

Peter Turecek

Hans-Peter Schwarz

Johann J Eibl

Bernhard Fischer

Falko-Guenter Falkner

Uwe Schlokat

Christa Tauer

Renate Den-Bouwmeester

Noel Barrett

Barbara Kraus

Simone Von Fircks

Hermann W Katinger

Otfried Kistner

Thomas R Kreil

Horst Schafhauser

Nicolaus Zach

Theodor Gaida

Gerald Bluml

Simone Feigl

Renata Baldaszti-Juric

Wolfgang Langer

Gregor Bramberger

Barbara Plaimauer

Markus Wolschek

Ales Strancar

Heidi Meyer

Bartold Rauschert

Mojca Tajnik Sbaizero

Axel Bruehmann

Roland Geyer

Leopold Gillberger

Renata Juric

Ruth Elmer

Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities.

Method of producing recombinant high molecular weight vWF in cell culture

The present invention relates to a method of predicting a performance characteristic of a plant or yeast hydrolysate, wherein a plant or yeast hydrolysate sample is measured with 2D fluorescence spectroscopy in powder form. Said method comprises the steps for providing a model based on a predetermined value of a manufacturing parameter of interest. For this purpose a training set consisting of predetermined manufacturing parameter of interest (e.g volumetric productivity parameter, virus titer or cell number) and fluorescence spectroscopic data is used. The fluorescence spectroscopic data is correlated to the values of the manufacturing parameter of interest to obtain a calibration model/model parameters by applying multivariate data analysis. This calibration model is being used to predict the manufacturing parameter of interest for new samples dedicated for the manufacturing process. This prediction is used for a decision to accept or reject the lot which corresponds to the respective sample for use in the manufacturing process or for further evaluation depending on the pre-defined range of the manufacturing parameter of interest. The invention further relates to a method for preparation of a cell culture medium, preferably an animal protein free cell culture medium, a method for cultivating cells, a method for producing a recombinant target protein, a method for producing an immunogenic composition, whereby the above method of predicting a performance characteristic has been used for selecting the plant or yeast hydrolysate to be used in the manufacturing process.

Method of predicting a performance characteristic of a plant or yeast hydrolysate and its use

The invention relates to a method for removing a viral contaminant from a preparation, being a cell culture medium or at least a component of a cell culture medium. The method comprises subjecting said preparation to filtration for at least about 24 hours through a virus filter having an effective pore size of maximum about 75 nm. Further, the invention relates to the use of a virus filter in filtration of at least about 24 hours, wherein the virus filter has an effective pore size of maximum about 75 nm for the removal of viral contaminant from a preparation, being a cell culture medium or at least a component of a cell culture medium. In some embodiments the filtration according to the invention operates at a volumetric capacity of at least about 2000 L/m. Further, the invention relates to the use of a preparation, being a cell culture medium or at least a component of a cell culture medium obtainable according to method of the invention for cell culture; pharmaceutical, diagnostic and/or cosmetic preparations as well as in food preparations.

Virus filtration of cell culture media

The present invention refers to a process for purifying virus particles from cell culture, comprising the steps of subjecting the cell culture to centrifugation to get a supernatant fraction of virus particles, incubating said fraction with a nuclease, diluting the fraction with low conductivity buffer, subjecting said diluted fraction to a SO3 chromatography step, performing a washing step with low conductivity buffer, and eluting virus particles.

SO3 chromatography for use in a method for virus purification

The present invention provides culture mediums that are useful for the expression of ADAMTS proteins, such as ADAMTS13. Methods for the expression and purification of ADAMTS proteins are also provided. In some embodiments, the mediums and methods of the invention are useful for the expression of ADAMTS proteins having high specific activities. Also provided are ADAMTS, e.g., ADAMTS13, protein compositions with high specific activities, which are expressed and purified according to the methods provided herein.

ADAMTS13 protein cell culture supernatant

Described herein is a chemostat-like continuous cell culture system that combines certain advantages of perfusion open systems and chemostat open systems to improve the culturing of mammalian cells, e.g., genetically modified cells, particularly in serum-free or chemically-defined media. The continuous culture system described herein involves culturing mammalian cells in a continuous cell culture system, which comprises a cell retention device, wherein the cell culture system has a dilution rate (D) of less than about 2 d, and a cell density of less than about 2&#xd7;10cell/mL. Also described herein is a method for producing a polypeptide and/or virus of interest in a continuous cell culture, the method comprising culturing mammalian cells expressing the polypeptide and/or virus of interest in a continuous cell culture system, which comprises a cell retention device, wherein the cell culture system has a dilution rate (D) of less than about 2 d, and a cell density of less than about 2&#xd7;10cell/mL; and recovering the polypeptide and/or virus of interest from medium of the cell culture system.

Method of producing a polypeptide or virus of interest in a continuous cell culture

Cell culture methods for expressing ADAMTS protein

The present invention relates to oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine and to methods for cultivating cells in said oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine. The invention also relates to methods for expressing at least one protein in a medium comprising at least 0.5 mg/L of a polyamine and to methods for producing at least one virus in a medium comprising at least 0.5 mg/L of a polyamine.

Oligopeptide-free cell culture media

The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.

Animal protein-free media for cultivation of cells

Cell culture methods for expressing ADAMTS13 protein

Growing community of inventors

Vienna, Austria

Manfred Reiter