Carl Zeiss Microscopy Gmbh

Carl Zeiss Jena Gmbh

Carl Zeiss Microimaging Gmbh

Manfred Matthae

Georg Herbst

Werner Kleinschmidt

Thomas Scheruebl

Norbert Czarnetzki

Bruene Venus

Andreas Faulstich

Lothar Schreiber

The invention relates to a planapochromatically-corrected immersion microscope objective for high-resolution microscopy applications with changing dispersive immersion conditions, having a plurality of lenses and/or subsystems (T, T, T) comprising lens groups and a corrective function (LA) for eliminating spherical aberrations. According to the invention, the microscope objective has an additional corrective function (LA) for eliminating longitudinal chromatic aberrations caused by dispersive changes in the immersion by changing the air gaps between the lenses or gap combinations, wherein the influence on the longitudinal chromatic aberration corresponds to a rotation of the curve s(&#x3bb;), which describes the color point (s) as a function of the wavelength (&#x3bb;).

Planapochromatically-corrected microscope objective

A device for the evanescent illumination of a sample, including an optical illumination element with an optical corrective element and an objective arranged downstream from the corrective element, to evanescently illuminate the sample with a supplied ray beam containing optical radiation with at least two different wavelengths. The corrective optical element has a transverse chromatic aberration which, during the illumination, leads to the optical radiation penetrating the pupil of the objective at different heights relative to the optical axis varying according to the wavelength. The corrective optical element is selected in such a way that the wavelength-related difference of the penetration depths of the radiation into the sample is reduced during the evanescent illumination.

Device and method for the evanescent illumination of a sample

A microscope objective with high aperture, large object field and apochromatic correction in the wavelength range from ultraviolet to infrared. The microscope objective includes, starting from the object level: a first group of lenses with overall positive refraction power, including a cemented group with positive-negative refraction power effect, made out of one of two lenses, and of a further lens with positive refraction power, a second group of lenses with positive refraction power, including three cemented lenses, a third group of lenses with negative refraction power, including three cemented lenses, in which the side that faces the image plane is convex, a fourth group of lenses, consisting of a lens with positive refraction power and a cemented group of two lenses with positive-negative refraction power, and a fifth group of lenses, including two lenses in a cemented group with negative-positive refraction power.

Apochromatically corrected microscope objective

A high-aperture objective comprising a first lens L with positive refractive power f and a second lens L with negative refractive power f wherein the focal length ratio between the two lenses is in the range of &minus;0.4<(f /f )<&minus;0.1 and the total refractive power /f /f is greater than zero, two positive lenses L L whose ratio diameter d d to focal length f f satisfies the condition greater than 0.3 and less than 0.6:0.3<d /f <0.6, 0.3<d /f <0.6, a negative lens L and a collective lens L wherein the negative lens faces the front group and the focal length ratio of L and L f /f is between &minus;0.5 and &minus;2:&minus;0.5<f /f <&minus;2.

High aperture objective lens

A microscope with incident light input coupling, wherein the light provided for the incident illumination is directed onto the partially reflecting layer of a beam splitter cube and is directed from there through the objective onto the specimen, while the light reflected and/or emitted by the specimen travels back to the partially reflecting layer and passes through the latter into the imaging beam path. In a microscope of this type, the beam splitter cube is provided with a negative spherical curvature at its outer surface facing the objective. Further, instead of the conventional tube lens, there is a combination formed of a converging lens and a diverging lens, wherein the surface curvatures of the converging lens and the diverging lens and the negative spherical curvature effected at the beam splitter cube are adapted to one another in such a way that the back-reflections of the incident illumination in the intermediate image plane are limited to a minimum.

Growing community of inventors

Jena, Germany

Manfred Matthae