Boehringer Mannheim Gmbh

Manfred Kuhr

Dieter Berger

Werner Guthlein

Gunter Frey

Wolfgang Werner

Franz Braun

Rupert Herrmann

Wolfgang-Reinhold Knappe

Walter Rittersdorf

Harvey Buck

Hans-Joachim Guder

Johann Berger

Hans-Georg Batz

Ulfert Deneke

Hans Wielinger

Felix Helmut Schmidt

Hartmut Merdes

Manfred Hubner

Herbert Buschek

Wolfgang Weckerle

Rudolf Machat

Wolfgang Kleeman

Hans-Rudiger Murawski

Vladimir Hrstka

Walter Aumuller

A process for the detection of substances with hydrolase activity in a sample by mixing the sample with a hydrolase substrate and an oxidising agent, and the evaluating the resultant colour intensity, wherein, as hydrolase substrate, there is used at least one compound of the formula ##STR1## in which R.sup.1 is hydrogen or an alkoxy radical,

Agent for the detection of substances with hydrolase activity

A process for the detection of substances with hydrolase activity in a sample by mixing the sample with a hydrolase substrate and an oxidizing agent, and the evaluating the resultant color intensity, wherein, as hydrolase substrate, there is used at least one compound of the formula ##STR1## in which R.sup.1 is hydrogen or an alkoxy radical,

Process for the detection of substances with hydrolase activity

Esters and agents including the esters, useful as substrates for esterolytic and/or proteolytic enzymes, said esters being compounds of the general formula: ##STR1## in which R'.sub.1, R'.sub.2, R'.sub.3 and R'.sub.4, which may be the same or different, are hydrogen atoms or lower alkyl, lower alkoxy, alkylamino or dialkylamino radicals or in which two adjacent substituents can also represent a fused benzene ring, A is a residue of an amino acid or of a peptide and B is a nitrogen protective group conventional in peptide chemistry or derived therefrom and the method of preparation and use.

Agent for the determination of esterolytic and/or proteolytic enzymes

The present invention provides phenolsulphonphthaleinyl-.beta.-D-galactosides of the general formula: ##STR1## wherein R.sup.1 to R.sup.4, which can be the same or different, are hydrogen or halogen atoms or nitro or amino groups, R.sup.5 to R.sup.12, which can be the same or different, are hydrogen or halogen atoms or lower alkyl, hydroxyl, lower alkoxy, carboxyl or nitro groups and M.sup.+ is a proton, an alkali metal, alkaline earth metal or ammonium ion. The present invention also provides a process for preparing these galactosides and diagnostic agents containing them.

Phenolsulphonphthaleinyl-.beta.-D-galactosides and diagnostic agents

The present invention provides imidazole derivatives of the general formula: ##STR1## wherein R, R.sub.1 and R.sub.2 are defined hereinbelow. The present invention is also concerned with processes for the preparation of these imidazole derivatives and with the use thereof as redox indicators.

Imidazole derivatives as redox indicators

Esters and agents including the esters, useful as substrates for esterolytic and/or proteolytic enzymes, said esters being compounds of the general formula: ##STR1## in which R'.sub.1, R'.sub.2, R'.sub.3 and R'.sub.4, which may be the same or different, are hydrogen atoms or lower alkyl, lower alkoxy, alkylamino or dialkylamino radicals or in which two adjacent substituents can also represent a fused benzene ring, A is a residue of an amino acid or of a peptide and B is a nitrogen protective group conventional in peptide chemistry or derived therefrom; and the method of preparation and use.

This invention relates to new amino acid and peptide esters of leuko-indoaniline compounds and to a process for their preparation. In additional aspect, the invention relates to compositions containing such compounds for the detection of proteolytic enzymes and to methods for detecting such enzymes.

Amino acid and peptide esters of leuko-indoaniline compounds and

Azo dyestuff for use as a chromogen in detecting leukocytes

This invention relates to diagnositc agents and methods for the detection of leukocytes in body fluids. In additional aspect, the invention provides a process for the production of such diagnostic agents.

Diagnostic agents for the detection of leukocytes in body fluids

Novel diagnostic composition for the detection of leukocytes in body fluids, comprising an absorbent carrier impregnated with a buffer substance and a chromgen, wherein the chromogen is an azo dyestuff ester of the formula

Diagnostic composition for the detection of leukocytes and chromogens

The present invention provides a diagnostic agent for the detection of proteolytic enzymes, comprising an absorbent carrier, a film layer, a powder mixture, a lyophilisate, a solution or a reagent tablet, containing at least one chromogen and an appropriate buffer substance, wherein the chromogen used is an indoxyl- and/or thioindoxyl-amino acid ester and/or peptide ester of the general formula: ##STR1## wherein R.sub.1, R.sub.2, R.sub.3 and R.sub.4, which can be the same or different, are hydrogen or halogen atoms, lower alkyl, lower alkoxy, aryl, aralkyl, aralkoxy, hydroxyl, carboxy, carboxy lower alkoxy, aralkoxycarbonyl, aralkyloxycarbonyl lower alkoxy, nitro or lower acylamino radicals, whereby two adjacent substituents can also represent an optionally halogenated fused benzene ring, X is a sulphur atom or an imino group optionally substituted by a lower alkyl, aryl, aralkyl or acyl radical, A is an amino acid or peptide residue and B is a nitrogen protective group conventional in peptide chemistry or derived therefrom. The present invention also provides new compounds of the above-given general formula and processes for the preparation thereof. Furthermore, the present invention is concerned with the use of the compounds of the above-given general formula for the production of diagnostic agents for the detection of proteolytic enzymes.

Diagnostic agents for the detection of proteolytic enzymes

Indole-carboxylic acid compounds of the formula: ##STR1## wherein R.sub.1 is lower alkyl or alkoxy radical or halogen;

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Mannheim, Germany

Manfred Kuhr