Max-planck-gesellschaft Zur F&#xf6;rderung Der Wissenschaften E. V.

Evotec Biosystems Ag

Evotec Biosystems Gmbh

Olympus Corporation

Evotec Oai Ag

Other

Manfred Eigen

Karsten Henco

Wolfgang Simm

Rudolf Rigler

Michael Stuke

Ulo Mets

Jerker Widengren

Michael Brinkmeyer

Detlev Riesner

Andreas Schwienhorst

Andre Koltermann

Jan Bieschke

Olaf Lehman

Christof Biebricher

Esteban Domingo

John Holland

Hajo Otten

Ulrich Kettling

Olaf Lehmann

Björn F Lindemann

Martin Pitschke

Oliver Schaefer

Karin Post

Bj&ouml;rn Lindemann

Thorsten Winkler

Jens Stephan

Petra Schwille

Klaus D&ouml;rre

Gunter Bauer

Roderich Weise

A Method for identifying one or a small number of molecules, especially in a dilution of &#x2266;1 &#x3bc;M, using laser excited FCS with measuring times &#x2266;500 ms and short diffusion paths of the molecules to be analyzed, wherein the measurement is performed in small volume units of preferably &#x2266;10l, by determining material-specific parameters which are determined by luminescence measurements of molecules to be examined. The device which can be preferably used for performing the method according to the invention is a per se known system of microscope optics for laser focusing for fluorescence excitation in a small measuring compartment of a very diluted solution and for imaging the emitted light in the subsequent measurement through confocal imaging wherein at least one system of optics with high numerical aperture of preferably &#x2267;1.2 N.A. is employed, the light quantity is limited by a confocally arranged pinhole aperture in the object plane behind the microscope objective, and the measuring compartment is positioned at a distance of between 0 and 1000 &#x3bc;m from the observation objective.

Method and a device for the evaluation of biopolymer fitness

A process for instabilizing viral quasi-species-distributions under avoidance of resistance phenomena by replication of the nucleic acids of the viruses present in the quasi-species-distribution by of a defective replication system,

Process and agent for instabilizing viral quasi-species-distributions avoiding resistance phenomena

The method according to the invention permits a selected withdrawal of one or a few molecularly disperse or cellular components of a system, such as molecules, molecular complexes, vesicles, micelles, cells, optionally together with an associated volume element V having a size of 10l&#x2267;V&#x2267;10l from a larger sample volume. The selected transfer of the sought component to another environment is effected by defining the space and time of withdrawal by means of a signal correlating with the small component to be withdrawn. The method is particularly useful for the withdrawal of non-abundant components the existence of which can be detected in a preceding step by a scanning process. The method is also useful for the withdrawal of per se unidentified components.

Method and device for the selective withdrawal of components from complex mixtures

Method for the production of biopolymers with modified properties

A method for identifying one or a small number of molecules, especially in a dilution of &lE;1 &mgr;M, using laser excited FCS with measuring times &lE;500 ms and short diffusion paths of the molecules to be analyzed, wherein the measurement is performed in small volume units of preferably &lE;10 l, by determining material-specific parameters which are determined by luminescence measurements of molecules to be examined.

Method for measuring the association of substructures of pathological protein depositions

The invention relates to a method for detecting reactions and conformational changes of analytes in a sample by coincidence analysis, as follows: the sample is marked with at least two different fluorescent dyes and then illuminated with at least one laser in order to stimulate the emission of fluorescence; the fluorescence signals are detected by at least two detection units and each signal is broken down into any simultaneous time segments with the desired time slot widths; the number of signals contained in at least one time segment and/or the time intervals between signals in the time segments are detected; a coincidence analysis of the data detected is carried out for at least one time segment of the first detection unit with at least one isochronous time segment of the second detection unit; statistics are produced showing the results of the coincidence analysis and/or the results are subjected to a threshold value analysis, and these statistics or a combination of more statistics are evaluated for the presence of characteristic features.

Method for detecting reactions by means of coincidence analysis

Process for the determination of in vitro amplified nucleic acids

Described is a support material that can simultaneously bind both genotypic and phenotypic substances. The respective areas, which can have surface-modifying matter such as anionic exchangers and/or affinity ligands, simultaneously bind, for example, nucleic acids and proteins or peptides. The support materials described can be used in processes for evolutive optimization of biopolymers, wherein genotype and phenotype can be bound at the same time so as to furnish them for further analysis.

Substrate material for simultaneously binding genotypic and phenotypic

A method for the direct identification of few, preferably single nucleic acid strands of a specific target sequence (10) in a test solution comprising the following steps:

Method for direct identification of few nucleic acid strands

The process of generating new biopolymers with improved characteristics, assisted by polymerases, is characterized in that at least one cycle of the steps described below, will be performed in a series of parallel preparations which are to be compared. Sequences of nucleic acids or, a mixture of similar sequences of nucleic acids in the distribution of mutants of a quasi-species, are subjected, in the region of the error threshold, to limited mutagenesis. These mixtures are replicated under simultaneous conditions and/or in succession. The mixtures of the so product nucleic acids are compartmentalized by segregation, and thereafter selected by a selection system which reflects the characteristics of interest of the nucleic acid sequence itself or, indirectly, via its translation product.

Process for manufacturing new biopolymers

A plate having wells therein for receiving chemical substances is provided with a coverplate covering said filled wells. The process for sealing the wells by providing a joining seam running around the wells provides the joining seam such that it has a profiled cross-section in order to increase the contact surface between the plate and the coverplate. To this end a welding die has a front surface provided with projecting profiled regions and being heated to a temperature in the region of the melting point of the coverplate. The welding die is placed on the coverplate means and remains there for a fixed first period. Then the welding die is pressed down through a fixed path and remains in this position for a fixed second period. Removing the welding die from the coverplate is the last step in the process.

Process for sealing at least one well out of a number of wells provided

Process of producing plastic plates with wells by placing a film on a

Growing community of inventors

Gottingen, Germany

Manfred Eigen