National Institute of Advanced Industrial Science and Technology

Secretary of Agency of Industrial Science and Technology

Manami Tanaka

Tomoo Tanaka

Masamitsu Yamaguchi

Provided is a specialstrain having a Bradeion gene artificially incorporated therein to exclusively express the gene in the compound eyes and thus to exhibit rough eye. Thestrain has the following properties:

Drosophila strain carrying bradeion gene(s) transferred thereinto

A human-derived bradeion protein, which has the following properties: (i) it is a transmembranous protein; (ii) it has a structure characteristic of growth hormone and cytokine receptors even in a structure of its transmembranous portion when its structure is determined by a hydrophobicity analysis according to Kyte-Doolittle method; (iii) it is expressed at a high level in a human adult brain, and in less amount in the heart, while it is not expressed in other adult organs or fetus; (iv) it induces programmed cell death (apoptosis) when over-expressed in a cultured human nerve cell lines; (v) it induces termination of cell division and aging when over-expressed in a cultured human normal cell; (vi) it is located in cytoplasm, and forms an intracellular aggregate when overexpressed; and (vii) besides human adult neurons, it is specifically expressed in a human colorectal cancer cell line or in a skin cancer cell line, or an analogue thereof.

Human-derived bradeion proteins, DNA coding for the proteins, and uses thereof

This invention relates to: a set of ribozymes which comprises at least one ribozyme capable of binding and cleaving Bradeion &#x3b1; mRNA which corresponds to DNA comprising a nucleotide sequence shown in SEQ ID NO:1, and at least one ribozyme capable of binding to and cleaving Bradeion &#x3b2; mRNA which corresponds to DNA comprising a nucleotide sequence shown in SEQ ID NO:3; a set of vectors comprising ribozymes; a pharmaceutical composition comprising set of vectors; and a method for treating a subject with a cancer using set of vectors.

Ribozymes targeting bradeion transcripts and use thereof

This invention relates to a human-derived bradeion protein having the following properties:

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Ibaraki, Japan

Manami Tanaka