Hitachi-high-technologies Corporation

Hitachi High-tech Corporation

Manami Nammoku

Kohshi Maeda

Yasunori Shoji

Ryoji Inaba

Toshinari Sakurai

Minoru Sano

Wataru Sato

Yoshiyuki Shoji

Terumi Tamura

Daisuke Morishima

Kimikazu Sugiyama

Yuki Kikuchi

Takehiko Hosoiri

The purpose of the invention is to provide a nucleic acid analyzer that sets and executes temperature regulation, said temperature regulation matching the characteristics of analyzing items and the configuration of the analyzer, by a simple operation while preventing deterioration in analytical performance caused by partial overheating of a liquid reaction mixture to thereby improve temperature change speed and shorten analysis time. To achieve the above purpose, provided is a method wherein, in the case of performing overshooting: as a first processing, the temperature is continuously elevated until reaching a target overshoot temperature; as a second processing, after reaching the aforesaid temperature, the overshoot target temperature is maintained for a preset period of time; and, as a third processing, the temperature is continuously lowered until reaching a target temperature of the liquid reaction mixture. By executing the first to third processings, regulation is conducted so that temperature measurement values are in a trapezoidal form.

Nucleic acid analyzer

When taking in outside air with a fan to control the temperature inside a covered portion where a specimen is installed, the internal temperature may change depending on an environment temperature to cause a difference in temperature control. The wind generated by the fan may blow against a reaction container depending on its loading position and it may influence the temperature control over individual specimens and lead to problems with temperature accuracy, temperature rise speed, and temperature drop speed. A reaction container avoiding these problems has a covered portion which performs temperature control on the reaction container and in which a cover and a fin cover have a heat insulating structure. A heat source is provided for controlling an internal temperature of an internal covered space. The internal temperature is kept constant and the influence of the environment temperature over the temperature control of the reaction container is minimized.

Nucleic acid amplification/detection device and nucleic acid inspection device using same

A nucleic acid analysis device is provided that is capable of quickly detecting device abnormalities, and the like. This device is provided with a temperature control block () for holding a tube () that includes a sample, a photometer (), and a device diagnosis unit. The photometer () is provided with an LED () for irradiating light toward the temperature control block () in a state in which the tube () is held and a fluorescence detector () for receiving light emitted by the sample in response to the irradiation of light from the LED (). The device diagnosis unit causes the LED () to irradiate light toward the temperature control block () in a state in which the tube () is not held, causes the fluorescence detector () to detect the resulting scattered light, and diagnoses the photometer () on the basis of the intensity of the scattered light.

Nucleic acid analysis device and diagnosis method

Growing community of inventors

Tokyo, Japan

Manami Nammoku