Ricoh Company, Ltd.

Manabu Seo

Kohei Shimbo

Naohiro Kamijo

Takahiko Matsumoto

Takeo Tsukamoto

Yoichi Kubota

Aino Hasegawa

Yuuma Usui

Daisuke Takagi

Hideomi Sakuma

Ryuya Mashiko

Yoshihiro Norikane

Hiroshi Akiyama

Hiroyuki Yamashita

Ryota Suzuki

Takeshi Orito

Hisayoshi Ohshima

Yuzuru Kuramochi

Yasufumi Yamada

Takeshi Hihara

Yoshio Uchikata

Hiroki Somada

Hideaki Hirai

Toshihiro Shigemori

Satoshi Nakazawa

Satoshi Izumi

Shigeo Takeuchi

Ikuo Katoh

Yudai Kawashima

Satoshi Okano

Goichi Akanuma

Mizuki Otagiri

Hidekazu Yaginuma

Shigeo Hatada

Koichi Muramatsu

Masahiro Fujimoto

Shigeru Oouchida

Yukiko Hamano

Nobuaki Toyoshima

Shusaku Shiomoto

Waka Lin

Hirotaka Unno

Masayuki Yumoto

Mayuko Murano

Hiroki Nakae

Yunong Ji

Provided is a nucleic acid sample-contained container including; a first nucleic acid molecule including an intended base sequence and a base sequence for detection different from the intended base sequence; and a second nucleic acid molecule free of the intended base sequence but including the base sequence for detection, wherein the nucleic acid sample-contained container includes the first nucleic acid molecule in a predetermined number. In a preferable mode, the copy number of the intended base sequence is less than 1,000, and the coefficient of variation (CV value) for the copy number is lower than 20%. In a more preferable mode, the nucleic acid molecules are artificially synthesized nucleic acid molecules. In a yet more preferable mode, the first nucleic acid molecule includes the intended base sequence in a plural number in the same molecule.

Nucleic acid sample-contained container, method and apparatus for producing nucleic acid sample-contained container, non-transitory recording medium storing program for producing nucleic acid sample-contained container, and nucleic acid sample

Provided is a method for producing a cell contained base, the method being capable of providing a cell contained base highly accurately controlled in number of nucleic acid molecules contained in a low-concentration nucleic acid standard sample, the method including a liquid droplet discharging step of discharging a cell suspension in the form of a liquid droplet with a liquid droplet discharging unit onto a base including at least one cell contained region; a cell number counting step of counting a number of cells contained in the liquid droplet with a plurality of sensors from two or more directions while the liquid droplet is flying into the cell contained region; and a liquid droplet landing step of landing the liquid droplet in the at least one cell contained region in a manner that a predetermined number of cells are located in the at least one cell contained region.

Method for producing cell contained base and method for evaluating equipment

Provided is a device including: at least one well in which an amplifiable reagent is contained in a specific copy number of less than 100; and at least one well in which the amplifiable reagent is contained in a specific copy number of 100 or greater, wherein for the at least one well in which the specific copy number of the amplifiable reagent is less than 100, a formula: CV&#x3c;1/&#x221a;x is established, where CV represents a coefficient of variation for the specific copy number and x represents average specific copy number of the amplifiable reagent.

Testing device for quantitative PCR

A droplet forming device is provided. The droplet forming device includes a liquid holder configured to hold a liquid, a film having a discharge hole, two or more vibration generators configured to vibrate the film, and a driver configured to apply a driving signal to the vibration generators. One or more of the vibration generators are disposed in each region on the film where a polarity of bending moment differs.

Droplet forming device, droplet forming method, and dispensing apparatus

Provided is discharging device including liquid droplet discharging unit configured to discharge liquid droplet containing a light-emitting particle that undergoes luminescence in response to irradiation of light, light irradiating unit configured to irradiate liquid droplet discharged by liquid droplet discharging unit with the light, light detecting unit configured to detect luminescence by light-emitting particle contained in liquid droplet in response to irradiation of the light emitted by light irradiating unit, and average particle number calculating unit configured to determine presence/absence of light-emitting particle in liquid droplet based on whether light detecting unit has detected luminescence by light-emitting particle, and calculate average particle number, which is average, among all liquid droplets discharged, in number of light-emitting particles contained per liquid droplet, based on ratio of liquid droplets containing no light-emitting particle relative to all the liquid droplets discharged, the ratio being calculated based on the presence/absence of light-emitting particle.

Discharging device and discharging method

A liquid droplet forming device includes a liquid droplet discharger that discharges, as a liquid droplet, a particle suspension liquid in which one or more fluorescent particles are suspended; a light source for irradiating light onto the flying liquid droplet discharged from the liquid droplet discharger; a photodetector that receives fluorescence that is emitted by the one or more fluorescent particles included in the liquid droplet in response to the light as excitation light; and a particle number detector that detects a number of the one or more fluorescent particles included in the liquid droplet based on information from the photodetector.

Liquid droplet forming device, dispensing device, and method of preparing base material

A particle counting apparatus is provided that includes: a droplet discharger configured to discharge a droplet containing at least one luminescent particle capable of emitting light upon receiving light; a light irradiator configured to irradiate the droplet discharged by the droplet discharger with light; at least one light receiver configured to receive light emitted by the at least one luminescent particle irradiated with the light emitted by the light irradiator; and circuitry configured to count luminescent particles contained in the droplet based on the light received by the at least one light receiver, the circuitry being configured to measure a presence or absence of the luminescent particles contained in the droplet; and to measure a number of the luminescent particles contained in the droplet.

Particle counting apparatus, particle counting method, and particle containing sample

A droplet forming apparatus includes a liquid retaining portion that retains cell suspension containing cells; a membrane member, provided with a nozzle, that discharges the cell suspension retained in the liquid retaining portion from the nozzle as a droplet by oscillation; and an open portion that opens the liquid retaining portion to atmosphere.

Droplet forming apparatus

A liquid droplet forming apparatus includes a liquid retention unit configured to retain particle suspension liquid in which precipitated particles are suspended; a membrane member, in which a nozzle is formed, configured to discharge the particle suspension liquid retained in the liquid retention unit, as a liquid droplet, by vibration from the nozzle; and a particle state detection unit configured to detect, from a side of the liquid retention unit, a particle state of the precipitated particle around the nozzle in the particle suspension liquid.

Liquid droplet forming apparatus

There is provided a liquid droplet forming apparatus comprising: a liquid holding part configured to hold a liquid including precipitating particles; a film member configured to be vibrated so as to eject the liquid held in the liquid holding unit, wherein a nozzle is formed in the film member and the liquid is ejected as a droplet from the nozzle; a vibrating unit configured to vibrate the film member; and a driving unit configured to selectively apply an ejection waveform and a stirring waveform to the vibrating unit, wherein the film member is vibrated to form the droplet in response to applying the ejection waveform and the film member is vibrated without forming the droplet in response to applying the stirring waveform.

A spectral characteristic obtaining apparatus including a light irradiation unit configured to emit light onto a reading object; a spectroscopic unit configured to separate at least a part of diffused reflected light from the light emitted onto the reading object by the light irradiation unit into a spectrum; and a light receiving unit configured to receive the diffused reflected light separated into the spectrum by the spectroscopic unit and to obtain a spectral characteristic. The light receiving unit is configured to be a spectroscopic sensor array including plural spectroscopic sensors arranged in a direction, and the spectroscopic sensors include a predetermined number of pixels arranged in the direction to receive lights with different spectral characteristics from each other.

Spectral characteristic obtaining apparatus, image evaluation apparatus and image forming apparatus

Using an intermediate transfer body onto which a conductive recording liquid containing water that is ejected by heads is provided; and a potential applying unit which applies a potential which can electrolyze the water within the conductive recording liquid which is ejected from the heads and which temporarily bridges between the heads and the intermediate transfer body, the potential applying unit applies a potential between the intermediate transfer body and electrodes which are in contact with the recording liquid in supplying units which supply the recording liquid to the heads.

Image forming apparatus

A spectral characteristic obtaining apparatus including a light irradiation unit configured to emit light onto a reading object; a spectroscopic unit configured to separate at least a part of diffused reflected light from the light emitted onto the reading object by the light irradiation unit into a spectrum; and a light receiving unit configured to receive the diffused reflected light separated into the spectrum by the spectroscopic unit and to obtain a spectral characteristic. In at least one example embodiment, the light receiving unit is configured to be a spectroscopic sensor array including plural spectroscopic sensors arranged in a direction, and the spectroscopic sensors include a predetermined number of pixels arranged in the direction to receive lights with different spectral characteristics from each other.

Disclosed is an aqueous ink composition, including an aqueous solvent, a coloring agent dissolved or dispersed in the aqueous solvent, an ABA-type amphipathic polymer including a hydrophobic segment A and a hydrophilic segment B, and an anionic surfactant configured to dissolve or disperse the ABA-type amphipathic polymer in the aqueous solvent, wherein a viscosity of the aqueous ink composition is changed depending on a pH thereof.

Aqueous ink composition and image forming apparatus

A spectrometer includes a light source to project a light beam to a target object, an optical element including a plurality of apertures through which the light beam reflected by the target object transmits, a diffraction element to form diffracted images from a plurality of light beams having transmitted through the optical element, and a light receiving element to receive the diffracted images formed by the diffraction element and including an optical shield to block a diffracted image other than a certain-order diffracted image.

Spectrometer, and image evaluating unit and image forming device incorporating the same

Growing community of inventors

Kanagawa, Japan

Manabu Seo