Korea Institute of Science and Technology

Man Ho Choi

Bong Chul Chung

Dong Hyun Kim

Ju-Yeon Moon

Seok Pil Lee

Jae Young Kim

Jung Hee Kim

Seung Ki Kim

Joon Seok Lee

Hyun Hwa Son

Chae Lin Lee

Eun Gyong Yoo

Hee Soo Pyo

The present invention relates to a method for evaluating metabolic activity based on the concentrations of cortisol and its precursors and metabolites thereof in saliva collected by a swab for the measurement of adrenal steroids relating to biochemical stress evaluation and more particularly, to a method of comparing metabolic activity of physiologically different adrenal steroids by analyzing adrenal steroids including cortisol and cortisone extracted from a trace amount of saliva by mass spectrometry and evaluating the metabolic ratio between the two compounds as well as the absolute amount of each compound. Accordingly, the present invention can be utilized as an index for evaluating response to biochemical stress and related diseases by measuring the amounts of adrenal steroids including cortisol and cortisone in a trace amount of saliva collected with a swab.

Method of evaluating adrenal steroids in saliva collected by cotton swab

Disclosed is a diagnostic kit for quickly diagnosing a target material with high sensitivity using nanoparticles that absorb infrared light and emit infrared light, in which the nanoparticles are maintained in particle size and have enhanced emission intensity.

Enhanced infrared ray absorbing/emitting nanoparticles and on-site diagnosis kit using same

Disclosed herein is a method for discriminating sitosterolemia by simultaneously measuring relative concentrations of phytosterol and cholesterol metabolism-related compounds in a blood spot on the paper. According to the present disclosure, sitosterolemia may be quickly discriminated with high accuracy by simple sample collecting using a trace amount of blood spot stained on a paper, and it may be used for newborn screening and preschool children, particularly patients with xanthoma.

Method for discriminatimg sitosterolemia using a dried blood spot

Provided is a method for selective extraction of steroid compounds using entrapped &#x3b2;-cyclodextrin polymers. Particularly, steroid hormones can be effectively selected from a biological sample by selectively extracting steroid compounds using entrapped &#x3b2;-cyclodextrin polymers, prepared by adding epichlorohydrin to &#x3b2;-cyclodextrin to prepare a polymer in a gel state, entrapping the polymer and pulverizing the products without using an additional device required in conventional solid-phase extraction.

Solid-phase extraction method of steroid hormones by entrapped &#x3b2;-cyclodextrin polymers

Provided are entrapped &#x3b2;-cyclodextrin polymers and a method of preparing the same. Particularly, entrapped &#x3b2;-cyclodextrin polymers are prepared by adding epichlorohydrin to &#x3b2;-cyclodextrin to prepare a polymer in a gel state, entrapping the polymer by entrapment of metal ions on the surface of the polymer and pulverizing the entrapped polymer to prepare powders. According to the entrapped &#x3b2;-cyclodextrin polymers, steroid hormones contained in a biological sample can be simply extracted without using an additional device required in conventional solid-phase extraction.

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Seoul, South Korea

Man Ho Choi