Bio-rad Laboratories, Inc.

Man Cheng

Yan Wang

Xiao-Song Gong

Rongdian Fu

John Sullivan

Evan H Bursey

Ronald Lebofsky

Adam McCoy

Xiangdong Meng

Kalyan Handique

Vishal Sharma

Andrew Kohlway

Jennifer Chew

Jan Zur Megede

Adam Mccoy

Zachary Burkett

Eric Pomaranski

Adam M Mccoy

Inventions covered include methods, systems, and compositions for sample processing, involving morphology-adjustable (e.g., tunable on-demand) functionalized particles. In some embodiments, a method can include distributing a set of functionalized particles, in a first morphological state, across a set of partitions; transitioning the set of functionalized particles, at the set of partitions, from the first morphological state to a second morphological state; transitioning the set of functionalized particles, at the set of partitions, from the second morphological state to a third morphological state, and inducing interactions between the set of functionalized particles and a set of targets, within the set of partitions and according to a set of operations with a set of process fluids.

Compositions, methods, and systems for sample processing with morphology-adjustable functionalized particles

Comparison of common sequencing reads from sequencing based on partition-based barcoding can be used to improve sequencing results. Increased loading of barcodes per partition can also improve sequencing results.

Nucleotide sequence generation by barcode bead-colocalization in partitions

Methods of detecting and amplifying short RNAs are provided.

RNA amplification methods

Mutant Type-A DNA polymerases having increased resistance to one or more polymerization activity inhibitors are provided.

Mutant DNA polymerases

The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonucleases activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.

Methods and compositions for improving efficiency of nucleic acids amplification reactions

Fusion polypeptides having a heterologous 5'-3&#x2032; exonuclease domain linked to a polymerase that does not naturally have 5&#x2032;-3&#x2032; exonuclease activity, as well as methods of their use are provided. Other aspects are also disclosed.

Fusion polymerases

Methods of determining polymerase fidelity are provided. In one embodiment, the method comprises filling a gapped plasmid with a polymerase to form a gap-filled plasmid, wherein the gap-filled plasmid comprises a gene encoding an protein that is functional or non-functional depending on the polymerase fidelity; forming a plurality of partitions from a solution comprising the gap-filled plasmid and a label for detecting the presence of the plasmid; detecting the presence of the gap-filled plasmid in one or more of the partitions; and determining the fidelity of the polymerase by determining a ratio of partitions containing the gene encoding a functional protein to partitions containing a gene encoding a non-functional protein.

Digital polymerase fidelity assay

Liquid storage solutions comprising polymerases are provided.

Arginine improves polymerase storage stability

The present invention provides methods for improving the specificity of nucleic acid amplification comprising incubating a nucleic acid molecule with a polymerase-Sso7 DNA binding domain conjugate and arginine, spermidine, or spermine. The present invention also provides reaction mixtures and kits for improving the specificity of nucleic acid amplification.

PCR reaction mixtures with decreased non-specific activity

Improved Sso7-polymerase conjugate proteins are provided.

Sso7-polymerase conjugates with decreased non-specific activity

The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonuclease activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.

Growing community of inventors

Danville, CA, United States of America

Man Cheng