Central Institute for Experimental Animals

University of Nebraska

Mamoru Ito

Naoki Yamamoto

Tatsutoshi Nakahata

Kiyoshi Ando

Kazuo Sugamura

Tatsuji Nomura

Koichiro Tsuji

Ikumi Katano

Kimio Kobayashi

Sonoko Habu

Yoshio Koyanagi

Larisa Poluektova

Santhi Gorantla

Daichi Nagai

Kazuya Okamoto

Ryoji Ito

The present invention provides a non-human animal having human interleukin-34 (IL-34) in the body thereof; a method for producing a non-human animal having human microglia, which includes transplanting human CD34-positive hematopoietic stem cells into the non-human animal having human IL-34 in the body; and a method for producing human microglia, which includes obtaining human microglia from the non-human animal having human microglia.

Non-human animal having human IL-34 and use Thereof

An object of the present invention is to provide a mouse that enables the functions of human NK cell to be studied. A DNA consisting of a nucleotide sequence represented by SEQ ID NO: 1, which is a gene region comprising a DNA in which a cDNA sequence encoding interleukin 15 (IL-15) is operably ligated to a cDNA sequence encoding the signal peptide of human interleukin (IL-2), is inserted to immunodeficient mouse cDNA. In NOD-scid, IL-2r&#x3b3;-hIL-15 Tg mice thus generated, hCD56cell having a concentration sufficient for conducting in vivo study on human mature NK cell are detected for at least 6 months after transplantation.

Human IL-15-secreting immunodeficient mouse

The present invention provides an immunodeficient mouse (NOG mouse) suitable for engraftment, differentiation and proliferation of heterologous cells, and a method of producing such a mouse. This mouse is obtained by backcrossing a C.B-17-scid mouse with an NOD/Shi mouse, and further backcrossing an interleukin 2-receptor &#x3b3;-chain gene-knockout mouse with the thus backcrossed mouse. It is usable for producing a human antibody and establishing a stem cell assay system, a tumor model and a virus-infection model.

[object Object]

Method of producing a mouse suitable for the engraftment, differentiation and proliferation of heterologous cells, mouse produced by this method and use of the mouse

Growing community of inventors

Kanagawa, Japan

Mamoru Ito