Millipore Corporation

Malcolm G Pluskal

Michael J Steuck

David Wang

James M Coull

John Charkoudian

Alex Garfield Bonner

Richard A Hamilton

Hubert Koester

Yolanda Nieuwkerk

Charles Phoebe

Robert J Barry

Darryl J Rappin

A method for covalent immobilization of a carbohydrate molecule with an oppositely charged surface, comprising the steps of adsorbing the carbohydrate molecule to the oppositely charged surface in proximity to reactive moieties bound to the charged surface; and next activating the bound moiety sufficiently for covalent attachment to the adsorbed carbohydrate molecule. Also disclosed is a hydrophobic microporous polymer membrane coated with a cross-linked, cationic polymer having fixed charges thereon, the coating having enhanced epoxide content for covalent immobilization of the oppositely-charged carbohydrate molecule. A novel method for sequencing carbohydrates by covalent attachment of a carbohydrate conjugate to the membrane, and subsequent treatment with glycosidases, is also presented.

Method and apparatus for covalent immobilization of charge- conjugated

The present invention relates to a device method and kit for the convenient and rapid isolation and purification of nucleic acids, proteins, peptides, carbohydrates and oligosaccharides from heterogeneous biological samples. The device comprises a membrane assembly comprised of layers of microporous, polymeric membranes functionalized with ion-exchange groups. The device is reusable for like samples, relatively inexpensive compared to currently available separation techniques and is disposable, thereby avoiding cross-contamination of biological samples.

Method for rapid purifiction of nucleic acids using layered ion-exchange

A membrane suitable for immobilizing peptides and proteins is disclosed. The membrane is a flexible, polymeric, porous membrane which contains functional groups capable of covalently linking the peptides and proteins. The functional groups can be provided by reacting the membrane itself or a coating thereon with nucleophiles which provide --NH.sub.2, --SH, --OH or --COOH functionality to the membrane surface. Additionally, surfaces containing --NH.sub.2 groups can be further reacted with diisothiocyantes to provide an isothiocyanate functionality having enhanced covalent binding characteristics.

Membranes for solid phase protein sequencing

A hydrophobic material having a crosslinked, cationic charge-modifying coating such that the majority of the ion exchange capacity of the material is provided by fixed formal positive charge groups is disclosed. The material is produced by contacting a hydrophobic substrate with a mildly alkaline, aqueous organic solvent solution into which has been dissolved a cationic charge-modifying agent. The charge-modifying agent comprises a water soluble, organic polymer having a molecular weight of greater than about 1000, wherein the polymer chain contains both fixed formal positive charge groups and halohydrin groups. Materials of the type described herein can be used in a variety of applications including macromolecular blotting and filtration.

Charge-modified hydrophobic membrane materials and method for making the

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Bedford, MA, United States of America

Malcolm G Pluskal