Kyowa Hakko Bio Co., Ltd.

Kyowa Hakko Kogyo Co., Ltd.

Makoto Yagasaki

Hajime Ikeda

Shin-ichi Hashimoto

Yoshiyuki Yonetani

Kuniki Kino

Kenji Soda

Keiichi Yano

Masato Ikeda

Kazuo Yamaguchi

Kazuhiko Tabata

Yoshiharu Yokoo

Yukio Hashimoto

Sadao Teshiba

Mikiro Hayashi

Tetsuro Ujihara

Masaru Hasegawa

Shuichi Ishino

Tetsuya Abe

Masaki Azuma

Yayoi Abe

Yuji Nakazawa

Jun-ichi Takano

Kazuhisa Iwata

Youichi Kotanaka

A target substance can be efficiently produced by culturing, in a medium, a coryneform bacterium in which the activity of a PTS protein relating to fructose uptake is reduced or lost as compared with a parent strain and the bacterium can produce the target substance, allowing the target substance to form and accumulate in a culture; and collecting the target substance from the culture.

Process for producing target substance by fermentation

The present invention provides: a protein having dipeptide-synthesizing activity; DNA encoding the protein; a recombinant DNA comprising the DNA; a transformant transformed with the recombinant DNA; a process for producing the protein having dipeptide-synthesizing activity using the transformant or the like; a process for producing a dipeptide using the protein having dipeptide-synthesizing activity; and a process for producing a dipeptide using, as an enzyme source, a culture of a transformant or a microorganism which produces the protein having dipeptide-synthesizing activity or the like.

Process for the production of dipeptides by a dipeptide-synthesizing enzyme

According to the present invention, a process for producing an amino acid using a microorganism in which aspartate aminotransferase activity is decreased or deleted, and which has the capability of producing and accumulating the amino acid is provided.

Method for producing L-amino acid using a microorganism with decreased aspartate aminotransferase activity

The present invention provides a process for producing a dipeptide or a dipeptide derivative using a phosphate donor, a substance selected from the group consisting of adenosine-5'-monophosphate, adenosine-5&#x2032;-diphosphate and adenosine-5&#x2032;-triphosphate, one or more kinds of amino acids or amino acid derivatives, and as enzyme sources, a protein having polyphosphate kinase activity, or a culture of cells having the ability to produce the protein or a treated matter of the culture, and a protein having the activity to ATP-dependently form the dipeptide or dipeptide derivative from one or more kinds of amino acids or amino acid derivatives, or a culture of cells having the ability to produce the protein or a treated matter of the culture.

Process for producing dipeptides or dipeptide derivatives

The invention provides a microorganism having an ability to produce a protein having a dipeptide synthesizing activity and in which an activity of the protein to transport a dipeptide in a microbial cell to theoutside of the microbial cell is higher than that of a parental strain.

Method for producing dipeptide

The present invention provides a protein having dipeptide-synthesizing activity, a DNA encoding the protein, a recombinant DNA containing the DNA, a transformant obtained via transformation with the recombinant DNA, a process for producing a protein having dipeptide-synthesizing activity using the transformant, a process for producing a dipeptide using a protein having dipeptide-synthesizing activity, and a process for producing a dipeptide using a culture or the like of a transformant or a microorganism that produces a protein having dipeptide-synthesizing activity as an enzyme source.

DNA encoding proteins having dipeptide-synthesizing activity and methods of using the same

The present invention provides a protein having low-substrate-specific amino acid racemase activity; DNA encoding the protein; a recombinant DNA comprising the DNA; a transformant carrying the recombinant DNA; a process for producing the protein by using the transformant; and a process for producing a racemic amino acid which comprises allowing a culture of the transformant or a treated matter thereof as an enzyme source and an amino acid to be present in an aqueous medium to racemize the amino acid in the aqueous medium, and recovering the racemic amino acid from the aqueous medium.

Low-substrate-specific amino acid racemase and process for producing racemic amino acid

Amino acid racemase having low substrate specificity and process for producing racemic amino acid

According to the present invention, by using a microorganism having the ability to produce the L-amino acid which carries the avtA gene encoding alanine-valine transaminase (transaminase C) and whose alanine-valine transaminase activity is enhanced compared with its parent strain, it is possible to reduce the production of by-product amino acids which disturb purification in L-amino acid production by fermentation, and an industrially advantageous process for producing an L-amino acid can be provided.

Process for producing L-amino acids via fermentation method

The present invention provides a DNA coding for uricase from the genus Cellulomonas and a process for producing uricase using a microorganism which carries a recombinant DNA containing the DNA.

DNA coding for uricase and process for producing uricase

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Tokyo, Japan

Makoto Yagasaki