Shiseido Company, Limited

The General Hospital Corporation

Koken Company, Limited

University School of Medicine

Makoto Tsunenaga

Eijiro Adachi

Masaru Suetsugu

Shinji Inomata

Kenichi Umishio

Shunsuke Iriyama

Toshio Nishiyama

Mikiko Kaminuma

Koji Kobayashi

Teruo Miyata

Robert E Burgeson

Masayasu Furuse

Naoki Tominaga

Masato Iino

Tomomi Furukawara

Chika Katagiri

Masahiro Ota

Nobuhiko Ochiai

Ken Kusakari

Nobuyuki Takakura

Nobuyuji Shioya

Sawako Ootake

Toru Yamashita

Mutsumi Fukuyama

Yoko Matsufuji

Hiroko Watanabe

Chieko Nomizu

The present invention relates to a method for producing artificial skin, comprising: adding a matrix metalloproteinase inhibitor and a heparanase inhibitor to an artificial skin formation culture medium comprising human epidermal keratinocytes and human dermal fibroblasts, culturing the cells in the artificial skin formation culture medium, and forming artificial skin.

Artificial skin

A vascular maturation, normalization or stabilization agent, or a Tie-2 activation agent, is provided comprising an extract derived from a plant belonging to the genus

Blood vessel maturation, normalization or stabilization agent and wrinkle preventer/improver

The present invention provides an agent for preventing and alleviating wrinkles which has excellent effects of preventing and alleviating wrinkles formed by decreased barrier function of the skin owing to aging or photo aging, that is graphic wrinkles or linear wrinkles. The agent for preventing and alleviating wrinkles consisting of one or more compounds selected from the group consisting of gamma-aminobutyric acid derivatives represented by the following formula (1) or N,N,N-trimethyl gamma-aminobutyric acid represented by the following formula (3) and salts thereof: wherein Rand Rindependently represent any of a hydrogen atom, an alkyl group having 1 to 3 carbon atoms, a benzyloxycarbonyl group, a cyclohexyl group, a cyclohexanemethyl group, a cyclohexanecarbonyl group, a guanidino group formed with nitrogen atom in the formula (1), an ureido group formed with nitrogen atom in the formula (1), and a group represented by following formula (2). Additionally, Rand Rmay jointly form a piperidine ring, morpholine ring, or pyrrolidine ring. Both of Rand Rare not simultaneously a hydrogen atom.

Agent for alleviating wrinkles

The present invention provides a composition for external application to skin containing one type or two or more types of compounds selected from the group consisting of aminoacetoamide compounds represented by the following general formula (1) and salts thereof. 

Composition for external application to skin

The use of laminin-5 to promote formation of basement membrane and to form skin equivalents and to improve grafting of keratinocyte sheets.

Laminin 5 and the formation of basement membrane structure

A hyaluronic acid-containing aqueous solution or aqueous dispersion of collagen having a pH of from about 6.5 to about 8.0 and an osmolality of from about 230 to about 320 mOsm/kgH.sub.2 O. The aqueous solution or the aqueous dispersion of collagen is useful as an agent for correction and reparation of a depressed part of or a void in soft tissue of mammals.

Hyaluronic acid-containing aqueous solution or aqueous dispersion of

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Yokohama, Japan

Makoto Tsunenaga