Japan Science and Technology Agency

The University of Tokyo

Makoto Oba

Kanjiro Miyata

Kazunori Kataoka

Nobuhiro Nishiyama

Yasutaka Anraku

Akihiro Kishimura

Tomoya Suma

Sayan Chuanoi

Satoru Matsumoto

Hideki Shimizu

HyunJin Kim

Yan Lee

Shinya Kaname

Shigehiro Hiki

Mai Sanjo

Yuichi Hori

Toshiro Fujita

Provided is a method for easily and efficiently producing encapsulated substance vesicles wherein a substance is encapsulated in the cavity of vesicles obtained by polymer self-assembly. Empty vesicles that have membranes comprising a first polymer that is a block copolymer with uncharged hydrophilic segments and a first kind of charged segments and a second polymer with a second kind of charged segments that carry a charge that is the opposite of said first kind of charged segments as well as spaces that are enclosed by said membranes are mixed in an aqueous medium with the substance that is to be encapsulated in the spaces.

Substance-encapsulating vesicle and process for producing the same

Disclosed is a polymer composite (polyplex) that contains nucleic acid, a cationic polymer, and an anionic polymer. The anionic polymer covers the surface of the composite comprising the cationic polymer and nucleic acid, has a negative charge at neutral pH, and can change so as to have a positive charge at mildly acidic pH.

Charge conversional ternary polyplex

Provided are a delivery system that is useful in delivering a double-stranded ribonucleic acid that functions in gene silencing in glomeruli, particularly in mesangial cells and the like, to the tissue or cells, and the like. A polyion complex in the form of a non-polymeric micelle consisting of a double-stranded ribonucleic acid and a block copolymer represented by the formula (I) or (II) below, which are electrostatically bound together, wherein the polyion complex has an average particle diameter of less than 100 nm as measured by a dynamic light scattering measuring method: wherein each symbol is as defined in the specification.

Polyion complex of double-stranded ribonucleic acid

Growing community of inventors

Tokyo, Japan

Makoto Oba