Tanabe Seiyaku Company, Limited

Makoto Masuda

Tetsuya Tosa

Takeji Shibatani

Taizo Watanabe

Naoki Sakurai

Saburo Komatsubara

Satoshi Minobe

Yuji Imai

There is disclosed a method for determining an endotoxin by reacting the endotoxin adsorbed on an adsorbent having specific endotoxin adsorbability with an endotoxin detecting reagent, including the steps of:

Method for determining endotoxin and apparatus therefor

A microorganism derived from a host microorganism capable of producing d-biotin by introducing a recombinant plasmid being incorporated with a biotin gene cloned from a microorganism of the genus Serratia capable of producing d-biotin and further integrating an exogenous biotin gene into the chromosome, and a process for preparing d-biotin which comprises cultivating the microorganism in a culture medium so that d-biotin is formed and accumulated in the culture medium and collecting the d-biotin. The microorganism of the invention has an extremely high productivity of d-biotin, and hence, d-biotin can be produced in a large amount by cultivating the microorganism of the invention.

Microorganism and process for preparing D-biotin using the same

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Takarazuka, Japan

Makoto Masuda