Ajinomoto Co., Ltd.

Api Corporation

Makoto Hibi

Jun Ogawa

Sakayu Shimizu

Yury Ivanovich Kozlov

Sergey Vasilievich Smirnov

Veronika Aleksandrovna Kotliarova

Tomohiro Kodera

Natalia Nikolaevna Samsonova

Natalia Yurievna Rushkevich

Hiroshi Kawabata

Ryoma Miyake

Vitaly Grigorievich Paraskevov, Legal Representative

The present invention provides a pipecolic acid 4-hydroxylase protein exemplified by the following (A), (B), and (C), having activity to react with L-pipecolic acid in the presence of 2-oxoglutaric acid and iron(II) ions to produce trans-4-hydroxy-L-pipecolic acid, and a method for producing 4-hydroxy amino acid, which method comprises reacting the pipecolic acid 4-hydroxylase protein, cells containing the protein, a treated product of the cells, and/or a culture liquid obtained by culturing the cells, with &#x3b1;-amino acid to produce 4-hydroxy amino acid:

Pipecolinic acid 4-hydroxylase and method for producing 4-hydroxy amino acid using same

A highly active L-isoleucine dioxygenase fromis provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.

Method for producing 4-hydroxy-L-isoleucine

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Kyoto, Japan

Makoto Hibi