Shimadzu Corporation

Other

Japan Science and Technology Corporation

The Institute of Physical and Chemical Research

Makoto Hazama

Shin Nakamura

Rintaro Yamamoto

Shinichi Utsunomiya

Yoshihide Hayashizaki

Kiyoshi Nokihara

Hideshi Fujiwake

Tetsuo Ohashi

Kei Kodera

Akira Harada

Toru Kaji

Hidesato Kumagai

Atsushi Inami

Keisuke Miyamoto

Takashi Ikegami

Naoya Endo

An ion trap includes: an ion trap including a plurality of electrodes; a rectangular voltage generator including a voltage source for generating a direct voltage and a switching section, the rectangular voltage generator configured to operate the switching section to generate a rectangular voltage by switching the direct voltage generated by the voltage source and to apply the rectangular voltage to at least one of the plurality of electrodes; and a switching section temperature controller configured to control a temperature of the switching section so as to maintain the temperature of the switching section at a target temperature which is higher than a highest reaching temperature of the switching section.

Ion trap device

Provided is an ion selection method capable of isolating and leaving a target ion in an ion trap within a short period of time and with high separating power. In a digital ion trap, after ions over a wide range of m/z near a target ion are selectively retained by rough isolation using an FNF signal or the like (S), unnecessary ions on a low-mass side are removed with high separating power by changing the duty ratio of a rectangular voltage (S). Furthermore, unnecessary ions on a high-mass side are removed with high separating power by resonant excitation discharge (S).

Ion selection method in ion trap and ion trap system

To enable accurate analysis of a base sequence even in an electrophoretic pattern containing a degraded part. The base sequence of a nucleic acid is determined by conducting the following steps (A) to (C) in this order: (A) a basic peak extracting step wherein basic peaks are extracted from electrophoretic data involving the respective peaks of the four bases obtained by electrophoresing a sample nucleic acid; (B) a condition determining step wherein a basic peak at the search starting point, from which the search is started, and a standard peak-to-peak distance are determined based on the time-series data composed of the basic peaks extracted above; and (C) a base sequence determining step wherein peak-to-peak intervals are successively scanned forward and backward in the above-described time-series data starting from the basic peak at the search starting point and then the peak-to-peak distance is compared with the standard peak-to-peak distance as determined above so as to add an interpolation peak to a peak-missing area.

Method of determining base sequence of nucleic acid

Disclosed herein is a method for assessing the degree of reliability of a nucleic acid base sequence, by which a higher-accuracy assessment result of the degree of reliability can be obtained as compared to a case where the degree of reliability is assessed based on only the evaluation of analytical data. The method includes the steps: (A) when measured data is processed into analytical data, computing a processing evaluation value E1for evaluating quality of measured data of an i-th base and contents of processing having been performed on the measured data of the i-th base; (B) computing an analytical data evaluation value E2of the i-th base based on the processed analytical data; and (C) computing a degree of reliability by a predetermined calculation formula using the processing evaluation value E1and the analytical data evaluation value E2.

Method for assessing degree of reliability of nucleic acid base sequence

For automating the operation of an electrophoresis apparatus and improving the throughput, the present electrophoresis apparatus has two platens capable of controlling temperature of electrophoresis plates placed thereon, a loading medium charging unit for sending a loading medium under pressure, a loading medium charging nozzle mechanism having a pair of nozzles connected to the loading medium charging unit, a pipetter mechanism for dispensing samples to sample dispensing openings of the electrophoresis plates placed on the platens, a stacker mechanism for storing sample plates, a loading buffer solution supplying mechanism, a loading buffer solution dispensing mechanism connected to the loading buffer solution supplying mechanism, a power unit for allowing electrophoresis separation for each electrophoresis plate placed on the platens, and a detector for optically detecting components migrating through each electrophoresis flow channel of the electrophoresis plates.

Electrophoresis apparatus

An electrode plate of a sample plate is set on the body of an electrophoretic apparatus, while a plug is inserted into a migration high voltage line connection hole and connected to a high-tension distribution cable. Each well of a base plate is inserted into a through hole of a well guide and further press-fit and engaged into a cavity of an electrode plate, for fixing the base plate to the electrode plate. Thereafter a sample is introduced into each well of the base plate and an end of a capillary column is dipped into each well for applying a migration voltage and electrophoretically injecting the sample into the capillary column.

Capillary electrophoretic apparatus, sample plate and sample injection method

Waveform shaping by Fourier transformation is performed on data of N points from the head of detected data with a parameter of a previously set peak interval (SS), base sequence is determined as to data of M points (M&#x3c;N) from the head of the data of N points (S), and a peak interval is obtained from the result of the sequence determination (S). Waveform shaping by Fourier transformation is performed on data of N points from a position returning by L points (L&#x3c;M) from final data of M points subjected to the sequence determination with a parameter of a precedently obtained peak interval, and thereafter the sequence determination, peak interval calculation and waveform shaping are similarly repeated. Thus, noise can be removed on the basis of Fourier transformation also as to a data section where a migration speed changes, for precisely determining the base sequence.

Method of determining a base sequence for nucleic acid

The present invention is directed to a simultaneous multiple chemical synthesizer comprising a number of reaction vessels wherein each vessel has a filter in the bottom portion thereof, a number of needles wherein each needle is connected to an aspiration injection line of a reaction mixture and a gas supply line in connection with each reaction vessel and each needle does not touch the resin contained in the reaction vessel, a number of arms which are horizontally and vertically movable and hold the respective needles, a bubbling gas line and a waste discharge line in connection with each reaction vessel, wherein each line is connected to the bottom portion of each reaction vessel, a number of purging means which move synchronously with said waste discharge lines, and means for washing the portions of the needles and purge means which contact with the reaction reagents.

Simultaneous multiple chemical synthesizer

In an automated solid-phase peptide synthesizing apparatus comprising at least one reaction vessel containing particulate resin as an insoluble support matrix which anchors elongating peptide chains yielded through coupling-assembly formation of peptide bonds, mixing agitation of reagents, as well as circulation agitation of washing solvents flushing the coupling-associated reaction solutions, introduced in peptide chain-assembly process steps into the reaction vessel, is effected by the bubbling of an inert gas forcibly passed through the reagents or washing solvent via a drainage port of the reaction vessel, in order to promote peptide synthesis. A removable agitation stabilizer is inserted into the reaction vessel as a barrier for inhibiting extra-vessel escape, due to action of the bubbling inert gas, of the reagents as well as the washing solvents, and of the support matrix anchoring the elongating peptide chains. The stabilizer also breaks clumping and coagulative skinning of elongated peptides bound to the support matrix, as effects further consequent of the action of the bubbling inert gas and levitated thereby toward the mouth of the reaction vessel during peptide synthesis. The stabilizer additionally acts as a baffle furthering mixing agitation, to promote dispersive mixing of the peptidyl resin with the reagents and washing solvents during peptide chain assembly; and It also serves to distribute washing solvents evenly along the inner surface of the reaction vessel.

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Kyoto, Japan

Makoto Hazama