Toyo Boseki Kabushiki Kaisha

Makoto Ando

Toshiro Kikuchi

Shigenori Emi

Yoshio Kojima

Masaru Ogawa

.alpha.-glycerophosphate oxidase is produced by cultivating microorganisms belonging to genus Pediococcus, Streptococcus, Lactobacillus or Leuconostoc in a nutrient medium containing at least one compound selected from the group consisting of .alpha.-keto acids represented by the formula,

Process for producing .alpha.-glycerophosphate oxidase

Novel acyl-coenzyme A oxidase is produced by cultivating an acyl-coenzyme A oxidase-producing microorganism belonging to the genus Candida in a nutrient medium, thereby accumulating an amount of acyl-coenzyme A oxidase in yeast cells and recovering the acyl-coenzyme A oxidase from said cells.

Process for producing acyl-coenzyme A oxidase

There is provided a method for the quantitative analysis of a free fatty acid which comprises (1) the first step of treating a sample containing the free fatty acid with acyl-coenzyme A synthetase in the presence of adenosine triphosphate and coenzyme A to form acyl-coenzyme A, (2) the second step of oxidizing said acyl-coenzyme A, in the presence of oxygen, with acyl-coenzyme A oxidase produced by a microorganism of the genus Candida to form enoyl-coenzyme A and hydrogen peroxide, and (3) the third step of (a) measuring the amount of the formed enoyl-coenzyme A or hydrogen peroxide or (b) measuring the amount of oxygen consumed in said oxidation reaction, to thereby determine the amount of free fatty acid in said sample.

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Tsuruga, Japan

Makoto Ando