The University of Tokyo

Tohoku University

Other

Japan Science and Technology Agency

Sekisui Medical Co., Ltd.

Nrl Pharma, Inc.

Mako Kamiya

Yasuteru Urano

Tetsuo Nagano

Toru Nakazawa

Keitaro Umezawa

Yugo Kuriki

Yuri Nagayo

Hiroki Ito

Daisuke Yamada

Kyoko Yamamoto

Hiroyuki Noji

Masafumi Yoshida

Kazuhito Tabata

Masayo Sakabe

Norihiro Kokudo

Takeaki Ishizawa

Haruki Kume

Hirohisa Iwaki

Minoru Kawatani

Kento Hayashi

Yu Kawamata

Suguru Yamashita

Tomohiro Doura

Akihiko Morozumi

Haruna Onoyama

Yasuyuki Seto

Shinnosuke Uno

Koushu Okubo

Junichi Hirahashi

As a technique for detecting an influenza virus with an improved accuracy, there is provided a method for detecting an influenza virus in a biological sample by using a first probe, which is decomposed by an influenza virus-derived neuraminidase and a bacterium-derived neuraminidase to generate an optically detectable signal, and a second probe, which is decomposed by the bacterium-derived neuraminidase to generate an optically detectable signal and not decomposed by the influenza virus-derived neuraminidase.

Method and kit for detecting influenza virus, and method for diagnosing influenza virus infection

A compound is represented by the following general formula (I): The compound or a salt thereof provides a fluorescent probe for detecting carboxypeptidase activity In the formula, Rrepresents an alkyl group having 1 to 6 carbon atoms or an alkoxy group having 1 to 6 carbon atoms; T represents an amino acid residue or a residue of an amino acid derivative; S represents a C-terminal amino acid residue. represents a fluorophore, and the fluorophore is a fluorophore in which an absorption/fluorescence wavelength greatly changes or a quenched state changes to a fluorescent state by elimination of the —P(═O)(—R)-T-S moiety.

Fluorescent probe for detecting carboxypeptidase activity

A fluorescent probe can be used to detect and visualize carboxypeptidase activity with high sensitivity. The fluorescent probe has, as a base nucleus, a fluorescent skeleton that functions in the visible light region, and makes carboxypeptidase activity detectable and visible, for example, within a cell or clinical specimen, with high sensitivity.

To provide novel compounds that are promising as prodrug-type anticancer agents, a compound represented by general formula (I) or a salt thereof is provided. 

Prodrug-type anticancer agent using cancer-specific enzymatic activity

[Problem] To provide a fluorescent probe that detects calpain activity in cells at high sensitivity. [Solution] A compound represented by the following general formula (I) or a salt thereof.

Fluorescent probe for detecting calpain activity

It is an object of the present invention to provide a fluorescence imaging probe capable of selectively visualizing target cells such as cells expressing &#x3b2;-galactosidase (lacZ expressing cells) at a single-cell level in a red fluorescence region, and of performing co-staining together with GFP. An intracellularly-retainable red fluorescent probe comprising a compound represented by the following formula (I) or a salt thereof: 

Enzyme-specific intracellularly-retained red fluorescent probe

[Problem] To provide a novel fluorescent probe for super-resolution imaging that uses fluorescent light emission characteristics that originate from an intermolecular nucleophilic addition-dissociation equilibrium reaction, and to provide a super-resolution fluorescent imaging method that uses the probe. [Solution] A fluorescent probe for super-resolution imaging that comprises a compound represented by formula (I) or a salt thereof [in the formula, X represents an oxygen atom, C(R)(R), or Si(R)(R) (wherein Rand Reach independently represent a hydrogen atom or an alkyl group), Rrepresents a hydrogen atom or an optionally substituted aryl (provided that, if Ris a phenyl group, the benzene ring of the phenyl group does not have a substituent at position 2 or position 6), Rand Reach independently represent 1-3 identical or differing substituents that are independently selected from the group that consists of hydrogen atoms, hydroxyl groups, halogen atoms, optionally substituted alkyl groups, optionally substituted sulfo groups, optionally substituted carboxyl groups, optionally substituted ester groups, optionally substituted amide groups, and optionally substituted azide groups, Rand Reach independently represent a hydrogen atom or an optionally substituted alkyl group or N(R)(R) forms an amide group or a carbamate group (provided that, if Ror Ris an alkyl group, each may form, together with R, a ring structure that contains the nitrogen atom that is bonded thereto), and Rand Reach independently represent a hydrogen atom or an optionally substituted alkyl group or N(R)(R) forms an amide group or a carbamate group (provided that, if Ror Ris an alkyl group, each may form, together with R, a ring structure that contains the nitrogen atom that is bonded thereto)], the fluorescent probe for super-resolution imaging being characterized in that the compound represented by formula (I) or the salt thereof undergoes a nucleophilic addition-dissociation equilibrium reaction with a nucleophilic compound that contains an &#x2014;SH group.

Super-resolution fluorescent imaging probe

[Problem] To provide a novel fluorescent probe for dipeptidyl peptidase IV, and a detection method and a detection kit using the fluorescent probe. [Solution] A fluorescent probe for detecting dipeptidyl peptidase IV (DPP-IV), said probe comprising a compound represented by formula (I) or a salt thereof. In formula (I): A and B are either the same or different and independently represent an amino acid residue, provided that A is bonded via an amide bond to NH in an adjacent formula and B is bonded via an amide bond to A; Rrepresents hydrogen atom(s) or 1 to 4 substituents bonded to the benzene ring, said substituents being either the same or different; R, R, R, R, Rand Rindependently represent a hydrogen atom, a hydroxyl group, an alkyl group or a halogen atom; Rand Rindependently represent a hydrogen atom or an alkyl group; and X represents a C-Calkylene group.

Fluorescent probe for detecting dipeptidyl peptidase IV

[Problem] To provide a fluorescent probe that detects calpain activity in cells at high sensitivity. [Solution] A compound represented by the following general formula (I) or a salt thereof. 

[Problem] To provide the following: a novel fluorescent probe for detecting a compound, such as glutathione, that contains a &#x2014;SH group; a detection method using said fluorescent probe; and a detection kit containing said probe. [Solution] A fluorescent probe for detecting a compound containing an &#x2014;SH group, wherein the fluorescent probe comprises a compound represented by formula (I) or a salt thereof (In the formula, X represents Si(R)(R), Ge(R)(R), Sn(R)(R), C(R)(R), or O (wherein Rand Reach independently represent a hydrogen atom or alkyl group); Rrepresents a hydrogen atom, or 1-4 identical or different substituents independently selected from the group consisting of a cyano group, alkyl group, carboxyl group, ester group, alkoxy group, amide group, and azide group, each of which may be optionally substituted; Rrepresents a hydrogen atom, halogen atom, hydroxyl group, cyano group, or an alkyl group, alkynyl group, alkoxy group, aryl, or heteroaryl, each of which may be optionally substituted; Rand Reach independently represent a hydrogen atom or 1-3 identical or different substituents independently selected from the group consisting of a hydroxyl group, halogen atom, or an alkyl group, sulfo group, carboxyl group, ester group, amide group and azide group, each of which may be optionally substituted; R, R, Rand Reach independently represent a hydrogen atom or alkyl group, wherein, Ror R, respectively together with R, may form a ring structure including the nitrogen atoms bonded thereto, or Ror R, respectively together with R, may form a ring structure including the nitrogen atoms bonded thereto).

Glutathione-detecting fluorescent probe

Provided is an enzyme-specific fluorescent compound capable of being retained in cells, which can emit fluorescence specifically in a target cell, particularly a cell capable of expressing a reporter enzyme such as &#x3b2;-galactosidase, and can covalently bind to a protein in the cell to exhibit an excellent property of being retained in the cell. The fluorescent compound comprises a compound represented by formula (I') or a salt thereof. In formula (I&#x2032;), A, X, Y and R1 to R9 are as described in claim

Enzyme-specific fluorescent compound capable of being retained in cells

A fluorescent probe for detecting pancreatic fluid containing a compound or salt thereof represented by formula (I) below. (In the formula, A represents an amino acid residue or N-substituted amino acid residue, with A forming an amide bond with the adjacent NH in the formula; Rrepresents a hydrogen atom or 1 to 4 same or different substituent groups that bond with a benzene ring; R, R, R, R, R, and Reach independently represent a hydrogen atom, a hydroxyl group, an alkyl group, or a halogen atom; Rand Reach independently represent a hydrogen atom or an alkyl group; and X represents a C-Calkylene group).

Fluorescent probe for high-sensitivity pancreatic fluid detection, and method for detecting pancreatic fluid

A fluorescent probe which is represented by the following formula (I): (wherein, Rrepresents a monovalent substituent other than hydrogen atom, carboxy group, or sulfo group; Rrepresents hydrogen atom, or a monovalent substituent; Rand Reach independently represents hydrogen atom or a halogen atom; and Rrepresents a monovalent group which is cleaved by contact with a measuring object, provided that a combination of Rand Ris selected so that the oxidation potential of the benzene ring to which they bind makes (1) the compound represented by the formula (I) substantially no fluorescent before the cleavage, and (2) a compound after the cleavage, which is derived from the compound represented by the formula (I), substantially highly fluorescent after the cleavage).

Growing community of inventors

Tokyo, Japan

Mako Kamiya