Konica Minolta, Inc.

Otsuka Pharmaceutical Company, Limited

Makiko Otani

Youichi Aoki

Tetsuya Noda

Fumio Nagai

Koji Miyazaki

A method for detecting an analyte according to the present invention includes: a first step of supplying a specimen to a detection device having a first ligand that is immobilized on a substrate and is capable of specifically binding to the analyte, the specimen being supplied onto the substrate of the detection device, and then causing the analyte included in the specimen to bind to the first ligand; a second step of supplying, onto the substrate, a second ligand that is labeled with a marker and is capable of specifically binding to the analyte, and then causing the second ligand to bind to the analyte bound to the first ligand; and a third step of measuring the second ligand bound to the analyte, wherein in the second step, carboxymethyl dextran is supplied onto the substrate.

Method for detecting analyte

A measurement chip including a prism, a metal film, and a capturing body is prepared. In a state in which a specimen is present on the metal film, scattered light obtained when first light which passes through the metal film and the specimen is scattered in the specimen when the first light is applied to the metal film from a prism side at a first incident angle smaller than a critical angle is detected. In a state in which a substance to be measured is captured by the capturing body and the specimen is not present on the metal film, a signal indicating an amount of the substance to be measured generated in the measurement chip when second light is applied to the metal film at a second incident angle not smaller than the critical angle from the prism side is detected. On the basis of a hematocrit value of the specimen determined from a light amount of the scattered light, a measurement value indicating the amount of the substance to be measured determined from the signal is corrected.

Growing community of inventors

Tokyo, Japan

Makiko Otani