Seikagaku Corporation

Maki Aizawa

Toshio Oda

Hiroshi Tamura

Shigenori Tanaka

Jun Aketagawa

Hikaru Mizumura

Koichi Makimura

A method for rapidly and highly sensitively measuring endotoxin relies on an endotoxin-measuring agent, which includes a factor C derived fromthat does not have His-tag sequence at the C-terminus, a factor B of a horseshoe crab, and a proclotting enzyme of a horseshoe crab. Each of these proteins can be a recombinant protein obtainable by being expressed using a stably expressing cell line of an insect cell as a host.

Method for producing factor C recombinant protein and endotoxin-measuring agent

An object of the present invention is to provide means for correctly measuring zygomycota. The present inventors have found that conventionally difficult zygomycota measurement can be performed through subjecting an untreated specimen to an acid treatment. The present invention provides a method for measuring zygomycota, the method including measuring zygomycota in a specimen that was subjected to an acid treatment; an agent for preparing a specimen for measurement of zygomycota; a method for preparing a specimen for measurement of zygomycota; and a reagent kit for measuring zygomycota.

Method for measuring zygomycota and reagent kit for measuring zygomycota

To provide means for reducing reaction interference observed when AT III is subjected totest, whereby thetest of antithrombin III can be carried out at high accuracy. Reaction interference observed when AT III is subjected totest can be reduced by subjecting AT III to a protein inactivation treatment in the co-presence of a divalent metal salt.

Pre-treatment agent and pre-treatment method for antithrombin III to be subjected to limulus test

The present invention relates to a process for preparing (1&rarr;3)-&bgr;-D-glucan, comprising subjecting a fungal cell to oxidation degradation under alkaline conditions to release (1&rarr;3)-&bgr;-D-glucan from a cell wall of the cell. (1&rarr;3)-&bgr;-D-Glucan can be prepared easily and efficiently from a cell of a microorganism belonging to the genus Candida. An accurate measurement process having a high reproducibility of (1&rarr;3)-&bgr;-D-glucan can be provided by using the above-described glucan or stabilized glucan into a kit and using it as a standard substance, and a large amount of the (1&rarr;3)-&bgr;-D-glucan having various biological activities can be solubilized and purified from a fungus, such as Candida or the like.

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Tokyo, Japan

Maki Aizawa