454 Life Sciences Corporation

Isoplexis Corporation

'454 Corporation

Roche Molecular Systems, Inc.

Maithreyan Srinivasan

Jonathan Marc Rothberg

John Harris Leamon

Michael Weiner

Yi-Ju Chen

Kenton L Lohman

Jan Berka

Vinod B Makhijani

Gary James Sarkis

Steve Lefkowitz

Steven M Lefkowitz

Sean MacKay

Patrick Paczkowski

Jonathan Rothberg

Jonathan M Rothberg

Colin Ng

Alaina Kaiser

Emily Bettini

Feimei Liu

Michael Reifler

Michael T Ronan

Sean Mackay

Aliana Kaiser

Kenton Lohman

John H Leamon

Disclosed are compositions and methods for the multiplexed analysis of one or more intracellular targets of a single cell. Exemplary compositions of the disclosure comprise a surface comprising a plurality of capture agents operatively-linked thereto, wherein each capture agent specifically binds to a distinct intracellular target and wherein the plurality of capture agents form a repeating pattern; a substrate comprising a plurality of chambers, wherein the substrate releasably couples with the surface and wherein each chamber of the plurality of chambers comprises at least one repeat of the repeating pattern of the plurality of capture agents of the surface; a coating composition comprising a cell lysis composition; and a linker composition comprising a functionalization component and an extension component.

Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells

Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.

Bead emulsion nucleic acid amplification

This invention relates to methods of sequencing DNA. More specifically, this invention relates to methods of sequencing both the sense and antisense strands of DNA through the use of blocked and unblocked sequencing primers. In brief, these methods include the steps of annealing an unblocked primer to a first strand of nucleic acid; annealing a second blocked primer to a second strand of nucleic acid; elongating the nucleic acid along the first strand with a polymerase; terminating the first sequencing primer; deblocking the second primer; and elongating the nucleic acid along the second strand.

Double ended sequencing

The present invention relates to the field of DNA recombinant technology. More specifically, this invention relates to fusion proteins comprising an ATP generating polypeptide joined to a polypeptide that converts ATP into a detectable entity. Accordingly, this invention focuses on sulfurylase-luciferase fusion proteins. This invention also relates to pharmaceutical compositions containing the fusion proteins and methods for using them.

Sulfurylase-luciferase fusion proteins and thermostable sulfurylase

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Branford, CT, United States of America

Maithreyan Srinivasan