Honda Motor Co., Ltd.

Maiko Tanaka

Shigenobu Mitsuzawa

Satoru Shinkawa

Masayuki Machida

Hiroko Hagiwara

Hideaki Koike

Mahoko Kuninaga

Daisuke Shibata

Takeshi Ara

Migiwa Takeda

Tomohiro Imai

Keiichi Kimura

To provide a base sequence for protein expression that can increase the yield of protein such as diastatic enzyme by further activating a promoter of a particular gene. A base sequencefor protein expression includes: a geneencoding protein; a promoterof the gene, the promoter being linked upstream of the gene; and a cis elementwhose activity is improved by an artificial transcription factor, the cis element being linked further upstream of the promoter. The cis elementis represented by SEQ ID NO: 1.

Base sequence for protein expression and method for producing protein using same

Nucleic acid sequence for protein expression

Anmutant strain characterized in that it is an auxotrophic mutant strain ofstrain AOK27L.

Aspergillus mutant strain

An object of the invention is to provide cellobiohydrolase with high activity. The activity of cellobiohydrolase can be improved by substituting, with alanine, asparagine that is the amino acid at position 62 from the N-terminal of cellobiohydrolase shown in SEQ ID NO: 1, or asparagine at a position corresponding to the position 62 in an amino acid sequence corresponding to the sequence of the cellobiohydrolase shown in SEQ ID NO: 1.

Method for producing highly active cellobiohydrolase and highly active cellobiohydrolase

Provided are anmutant strain which can dramatically enhance production of a heterologous enzyme when a saccharifying enzyme gene is transferred into the strain to perform transformation and a transformant having a saccharifying enzyme gene transferred into themutant strain. Themutant strain has been completely or partially deficient in three genes ofstrain HO2 (accession number: NITE BP-01750): a prtR gene coding for a transcription factor; a pepA gene coding for an extracellular acid protease; and a cpI gene coding for an extracellular acid carboxypeptidase.

Aspergillus mutant strain and transformant thereof

The present invention relates to a polypeptide which has &#x3b2;-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 91% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel &#x3b2;-glucosidase enzyme derived from, a polynucleotide encoding the &#x3b2;-glucosidase, an expression vector for expressing the &#x3b2;-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the &#x3b2;-glucosidase can be provided.

&#x3b2;-glucosidase

The present invention relates to a polypeptide which has &#x3b2;-glucosidase activity, and which includes an amino acid sequence represented by SEQ ID NO: 1, a polypeptide including an amino acid sequence in which one or several amino acids are deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, or a polypeptide including an amino acid sequence having 90% or greater sequence identity with the amino acid sequence represented by SEQ ID NO: 1. According to the present invention, a novel &#x3b2;-glucosidase enzyme derived fromcellulolyticus, a polynucleotide encoding the &#x3b2;-glucosidase, an expression vector for expressing the &#x3b2;-glucosidase, a transformant incorporated with that expression vector, and a method for producing a cellulose degradation product using the &#x3b2;-glucosidase can be provided.

Anmutant strain obtained by deleting a ligD gene from an auxotrophic mutant strain ofstrain AOK27L.

Growing community of inventors

Saitama, Japan

Maiko Tanaka