Qiagen Gmbh

Qiagen Gmgh

Maiko Lohel

Thorsten Zerfass

Björn Labitzke

Guido Schuster

Thomas Unterer

Fernando Carrillo Oesterreich

Jan Hauth

Thorsten ZERFAß

Fernando Oesterreich Carrillo

Method and system for compensating intensity biases in a plurality of digital images. Each digital image of the plurality of digital images contains a plurality of objects and each of the plurality of objects is configured to receive at least one molecule comprising genetic information, wherein the at least one molecule is configured to receive one of at least a first fluorescent compound and a second fluorescent compound. A first digital image of the plurality of digital images is taken by an optical imaging system during emission of electromagnetic radiation by the first fluorescent compound, and a second digital image of the plurality of digital images is taken by the optical imaging system during emission of electromagnetic radiation by the second fluorescent compound.

Cross-talk compensation

The invention relates to a method of neighbor influence compensation between a plurality of objects in at least one digital image, wherein the at least one digital image contains image information about a plurality of objects. Each of the plurality of objects is configured to receive at least one molecule comprising genetic information, wherein the at least one molecule is configured to receive a fluorescent compound, and the at least one digital image is taken by an optical imaging system during emission of electromagnetic radiation of the fluorescent compounds received by the at least one molecules.

Neighbor influence compensation

The present invention relates to a method and a system for aligning at least one part of a second digital image with at least one part of a first digital image comprising: dividing the at least one part of the first digital image into a plurality of tiles t_fi; dividing the at least one part of the second digital image into a plurality of tiles t_si; creating a plurality of correlation images fci; determining the position of a global maximum correlation value p_&#x3bd;' for each of the plurality of correlation images fci_&#x3bd;&#x2032;; calculating a plurality of first offset vectors fov, the offset vector fov_&#x3bd;&#x2033; representing the offset between the tile t_si&#x3bd;&#x2033; and the corresponding tile t_fi&#x3bd;&#x2033;; and aligning the at least one part of the second digital image with the at least one part of the first digital image by applying the corresponding first offset vectors fov.

Method for aligning at least one part of one digital image to at least one part of another digital image

The present invention relates to a method and a system for the overall brightness of at least one object in a digital image. In particular, the method for determining the overall brightness of at least one object in a digital image, the at least one object being configured to receive molecules comprising genetic information, the method comprising the steps of: generating an area A comprising k sample points, the k points being distributed in the area A according to a predetermined distribution; aligning the center position of the area A to the center position of the at least one object in the digital image; extracting the intensity value at each of the k sample points; and calculating the central tendency of the extracted intensity values at the respective k sample points.

Method for determining the overall brightness of at least one object in a digital image

System, method and computer program product for background compensation of a digital image containing at least one first object, the at least one first object having received at least one molecule comprising genetic information, and at least one second object, the at least one second object not having received a molecule comprising genetic information, the at least one molecule being configured to receive a fluorescent compound, the digital image being determined by an optical imaging system during emission of electromagnetic radiation by the fluorescent compound.

Background compensation

The present invention relates to a method and a system for determining the positions of a plurality of objects in a digital image by discriminating true positive positions of the plurality of objects from false positive candidate positions of the plurality of objects. In particular, the invention relates to a method for determining the positions of a plurality of objects in a digital image by discriminating true positive positions of the plurality of objects from false positive candidate positions of the plurality of objects, the plurality of objects being configured to receive molecules comprising genetic information.

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Hilden, Germany

Maiko Lohel