Rohm Enzyme Finland Oy

Rohm Enzyme Gmbh

Maija-Leena Onnela

Merja Elisa Penttila

Marja Hannele Ilmen

Tiina Hannele Nakari

Marja Ilmen

Kaisu Milja Nevalainen

A method is described for the identification and cloning of promoters that express under a defined environmental condition, such as growth in glucose medium. Using this method, five Trichodermal promoters capable of the high expression of operably linked coding sequences are identified, one of which is the promoter for T. reesei tef1. Also provided are altered cbh1 promoters, altered so that glucose no longer represses expression from such promoter. The invention further provides vectors and hosts that utilize such promoters, and unique fungal enzyme compositions from such hosts.

Fungal promoters active in the presence of glucose

Shortened sophorose inducible promoters are provided. The invention further provides vectors containing such promoters hosts transformed with the same, and methods utilizing such promoters for the expression of operably linked sequences.

Promoters and uses thereof

Method for cloning active promoters

Growing community of inventors

Helsinki, Finland

Maija-Leena Onnela