Toray Industries, Inc.

Mai Yagi

Masateru Ito

Yoji Ueda

Yuki Takii

A method evaluates a quality of a dephosphorylation reagent, the method including the steps of: providing a dephosphorylation reagent containing an alkaline phosphatase and a peptide fragment derived from the alkaline phosphatase; and evaluating the dephosphorylation reagent as having a high quality if a content ratio of the peptide fragment to the alkaline phosphatase is a predetermined reference value or less.

Method of evaluating quality of dephosphorylation reagent and method of detecting target nucleic acid

A composition contains an alkaline phosphatase and first to sixth peptide fragments, wherein content ratios of the first to sixth peptide fragments to the alkaline phosphatase satisfy formulas (1) to (6), respectively: (X/Y)×100≤0.6000 (1); (X/Y)×100≤0.1800 (2); (X/Y)×100≤0.2000 (3); (X/Y)×100≤0.8000 (4); (X/Y)×100≤1.6000 (5); and (X/Y)×100≤0.3500 (6), wherein Xto Xrepresent peak area values of the first to sixth peptide fragments calculated by an automatic integration method from an extracted ion chromatogram obtained by an LC-MS/MS analysis of the composition, respectively, and Y represents a peak area value of the alkaline phosphatase calculated by an automatic integration method from a chromatogram obtained by an LC-UV analysis of the composition.

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Kamakura, Japan

Mai Yagi