Universiti Putra Malaysia

Malaysian Palm Oil Board

University Putra Malaysia

Universiti Purta Malaysia

Mahiran Basri

Abu Bakar Salleh

Raja Noor Zaliha Abd Rahman

Mohd Basyaruddin Abd Rahman

Raja Noor Zaliha Raja Abdul Rahman

Raja Noor Zaliha Raja Abd Rahman

Mohd Zobir Hussein

Halila Jasmani

Siti Salhah Othman

Salmiah Ahmad

Roila Awang

Izuddin Abdul Rahman

Leow Thean Chor

Raja Nor Zaliha Raja Abdul Rahman

Noraini Abd Ghani

Mohd Basyaruddin Abdul Rahman

Thean Chor Leow

Raja Noor Zaliha Raja Abdul

Mohm Basyaruddin Abd Rahman

Nur Fariza Abd Rahman

Siti Salwa Abd Gani

Mohd Shukuri Bin Mohamad Ali

Raja Noor Zaliha

Randa Abdelkareem Abusham

Adam Leow Thean Chor

Raja Noor Zaliha RajaAbd Rahman

Afshin Ebrahimpour

Noraini Abdul Ghani

Jalimah Binti Yunus

The objective of the present invention is in the field of cleaning agent in particular detergents. In particular, it relates to a novel detergent formulation for an automatic dishwashing. The formulation provides excellent cleaning and finishing; it is environmentally friendlier than traditional compositions and allows for a more energy efficient automatic dishwashing process.

Detergent formulation for dishwashing machine

A method for crystallizing astrain T1 polypeptide under gravity or microgravity conditions is provided. A purified protein to be crystallized is provided, the purified protein being either thermostable T1 lipase or thermostable F16L lipase. A protein solution is prepared, comprising the purified protein, a buffer solution having a working pH of at least 8.5, and between 3-5% glycerol. A reservoir solution is prepared, comprising 0.1M MES, 0.1M NaH2PO4, 0.1M KH2PO4, and 1 M NaCl, and having a working pH of 6.5 or 6.6. The protein and reservoir solutions are combined to obtain a protein droplet, and protein crystals are obtained from the protein droplet by hanging-drop vapor diffusion.

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The present invention relates to a thermostable protease useful as an enzyme for industrial use, a gene encoding the same and a method of producing the enzyme by genetic engineering technique. More particularly, the present invention discloses a thermostable organic solvent tolerant protease and its code gene and application. The invention also discloses a method for preparing protease by isolating fromisolate Rand bacteria. The activity and stability of protease (preferably named Rand protease) at high temperature, and can be used in fields of washing agent industry, foodstuff industry, biological pharmacy and environmental biological technique.

Thermostable organic solvent tolerant protease from gram-positive bacteria

The objective of the present invention is to obtain a low temperature producing enzyme and more particularly, the enzyme provides a bifunctional purpose of varying its enzyme activity into activity of another enzyme. The present invention has overcome problems such as ability of obtaining an enzyme at low temperature.

Cold active enzyme and method thereof

An isotropic or homogeneous emulsion system comprises an oil phase including fat esters of illipe fat and a solubilizer; a non-ionic surfactants; and deionized water; characterized in that the solubilizer is a mixture of polyethyleneglycol-40 hydrogenated castor oil, polysorbate 20 and octoxynol-12.

Emulsion system derived from engkabang fat esters

The present invention provides a method of crystallizing of enzymes. The method is for rapidly crystallizing enzymes from impure mixtures. The method is simple and cheap, and it is compatible to industrial requirements. T1 lipase was able to form crystals at low protein concentration (2.5 mg/ml) in a day. High temperature crystallization was obtained from the method. The present invention also relates to a composition of a crystallized lipase produced from the said method.

Immobilization oflipase on a carrier selected from the group consisting of macroporous adsorbent resin of the acrylic type, synthetic epoxy activated resin and Mg&#x2014;Al-hydrotalcite enhances its enantioselectivity by six to seven folds. The immobilizedlipase is suitable for use in resolution of racemic alcohols and/or carboxylic acids, particularly in resolution of racemic menthol or production of menthyl esters.

Method of producing enantioselective immobilized candida rugosa lipase

The present invention relates to a Solvent-free coating material and treating agents used as wood coating material as a protective agent. The wood coating material is used to provide a short period of time ultra-violet (UV) radiation curing. Furthermore the coating material relates to an anticorrosion formulation and a method for forming a coated panel. This invention also provides a low volatility and high scratch resistance of coating for wooden surfaces.

Formulation for coating material

Wax esters are prepared by reacting a solution of palm oil in an organic solvent with oleyl alcohol in the presence of an immobilized lipase wherein molar ratio of palm oil to oleyl alcohol is between 1:2 and 1:4. Immobilized lipase used is present in an amount which is equivalent to not less than 1000 &#x3bc;g of protein per milli-mole of palm oil used and the reaction is carried out at 40&#xb0; C. to 50&#xb0; C. for a period of not less than 5 hours. The organic solvent used has a value of log P not less than 3.5.

Production of wax esters

Enantioselective immobilized lipase

A biologically pure culture ofStrain T1 bacteria isolated from Palm Oil Mill Effluent capable of producing thermostable lipase T1 gene. The production of thermostable lipase T1 gene from a novelbacterium having a designated as strain T1, was aerobic, gram positive and endospore-forming, rod-shaped. The G+C content of the genomic DNA was 52.6%. On the basic of physiological data, phenotypic traits and molecular analysis, strain T1 represents a novel species within the genus, The thermostable T1 lipase gene was subcloned into the pGEX-4T1 vector with and without signal peptide in prokaryotic system. The 28 amino acid residues signal peptide alter the conformation of GST moiety and preventing it from bind to affinity glutathione Sepharose column. By expression and simplification of the purification through single step affinity chromatography shows a specific activity of 292.929 U/mg and 72.55% of fusion lipase was recovered from crude cell lysate. T1 mature lipase was successfully purified with a final recovery of 51.49% and specificity activity of 959.033 U/mg.

A process for producing wax ester comprising of esterifying dihydroxy fatty acid preferably dihydroxystearic acid with at least one alcohol in the presence of lipase as a catalyst which is then remove by filtration. The filtered catalyst could be reuse and provides a number of cost advantages. In the preferred embodiment of the present invention, the dihydroxystearic acid is derived from palm based oleic acid and the alcohol contains 8 to 18 carbon atoms per molecules.

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Selangor, Malaysia

Mahiran Basri