Aarhus Universitet

Other

The Chinese University of Hong Kong

Zymonostics Aps

Magnus Stougaard

Birgitta Ruth Knudsen

Yi-Ping Ho

Kam W Leong

Christian Kanstrup Holm

Yonglun Luo

Marianne Smedegaard Hede

Charlotte Thornild Møller

Sissel Juul

Mette Nyegaard

Jørn Erland Koch

Felicie F Andersen

Birgitta R Knudsen

The present invention relates to a composition comprising a first gRNA bound to an RNA-guided endonuclease forming a first ribonucleoprotein complex, said first gRNA comprises a first targeting sequence being complementary for a first target sequence in an intron in the 5' upstream part of a fusion gene; and a second gRNA bound to an RNA-guided endonuclease forming a second ribonucleoprotein complex, said second gRNA comprises a second targeting sequence being complementary for second target sequence in an intron in the 3′ downstream part of the fusion gene. Such compositions may find use in the treatment of fusion gene related cancers.

CRISPR gene therapy of fusion gene related cancers

The present invention relates to methods for determining endonuclease activity in a sample. In particular, the present invention relates to a method for determining viable pathogenic bacteria in a sample based on patterns of endonuclease activity.

Detection of endonuclease activity

Methods for the identification of microorganisms or infectious disorders are disclosed, comprising obtaining a suitable sample from sources such as persons, animals, plants, food, water or soil. The methods also comprise providing tailored nucleic acid substrate(s) designed to react with a type 1 topoisomerase from one or more microorganism(s) or infectious agent(s), and incubating said substrate with said sample, or extracts or preparations from the sample, so that the substrate is processed by said topoisomerase if said microorganism(s) or infectious agent(s) is present in the sample. Microfluidic-implemented methods of detecting an enzyme, in particular a DNA-modifying enzyme, are also provided, as well as methods for detecting a cell, or a microorganism expressing said enzyme. The enzyme is detected by providing a nucleic acid substrate, which is specifically targeted by that enzyme.

System for identification of microorganism and detection of infectious disease

Growing community of inventors

Højbjerg, Denmark

Magnus Stougaard