Biacore Ab

Ge Healthcare Bio-Sciences Ab

Medical Research Council

Pharmacia Biosensor Ab

Cambridge Antibody Technology Limited

Ridgeview Instruments Ab

Ridgeview Diagnostics Ab

Magnus Malmqvist

Mattias Tidare

Gregory Paul Winter

James David Marks

Andrew David Griffiths

Brian Timothy McGuinness

Stefan Sjolander

Anthony Richard Pope

Hakan Erik Roos

Ralph Stalberg

Terence Derek Prospero

Karl Andersson

Stefan Sjölander

Hendricus Renerus Jacobus Matheus Hoogenboom

Robert Karlsson

H&aring;kan E Roos

Kaspar-Philipp Holliger

Håkan Sjödin

Anita Larsson

H&aring;kan Sj&ouml;din

Hendricus Renerus Hoogenboom

Stefan Sj&ouml;lander

Inger Ronnberg

Markku H&auml;m&auml;l&auml;inen

Östen Jansson

Håkan Roos

Bjorn Persson

Hakan Sjödin

Ralph St{dot Over (a)}lberg

Jorgen Sjodal

Ralph St&aring;lberg

Kaspar-Philip Holliger

&Ouml;sten Jansson

Ralph Stålberg

Lars Gedda

Lena Lebel

Hanna BJÖRKELUND

A method of analysis or diagnosis of solid objects is based on real-time detection of how predefined probes interact with structures present on or in the solid object combined with the calculation of how the recorded binding curves of said probes are distributed in terms of interaction properties. The interaction properties are input to a classification algorithm which automatically determines statues of the solid object. The method is particularly advantageous for solid biological objects like tissue slices combined with antibody probes, said antibody recognizing receptors known to be over-expressed in disease states on said tissue slice.

Method for the analysis of solid objects

A method for quality control of species used in analytical or diagnostic or therapeutic procedures includes immobilization of a model of the malignancy to a solid support (121), contacting the solid support with species dissolved in liquid (122), measuring both the rate of formation of complex and absolute magnitude of number of complexes of model and species (123) and determining the quality of species by comparing the measured values with predetermined values.

Method for the quality control of molecules or targets

Methods and devices are provided for controlling a fluid flow over a sensing surface within a flow cell. The methods employ laminar flow techniques to position a fluid flow over one or more discrete sensing areas on the sensing surface of the flow cell. Such methods permit selective sensitization of the discrete sensing areas, and provide selective contact of the discrete sensing areas with a sample fluid flow. Immobilization of a ligand upon the discrete sensing area, followed by selective contact with an analyte contained within the sample fluid flow, allows analysis by a wide variety of techniques. Sensitized sensing surfaces, and sensor devices and systems are also provided.

Method and device for laminar flow on a sensing surface

Polypeptides comprising a first domain, which comprises a binding region of an immunoglobulin heavy chain variable region, and a second domain, which comprises a binding region of an immunoglobulin light chain variable region, the domains being linked but incapable of associating with each other to form an antigen binding site, associate to form antigen binding multimers, such as dimers, which may be multivalent or have multispecificity. The domains may be linked by a short peptide linker or may be joined directly together. Bispecific dimers may have longer linkers. Methods of preparation of the polypeptides and multimers and diverse repertoires thereof, and their display on the surface of bacteriophage for easy selection of binders of interest, are disclosed, along with many utilities.

Multivalent and multispecific binding proteins, their manufacture and use

Methods and devices are provided for controlling a fluid flow over a sensing surface within a flow cell The methods employ laminar flow techniques to position a fluid flow over one or more discrete sensing areas on the sensing surface of the flow cell. Such methods permit selective sensitization of the discrete sensing areas, and provide selective contact of the discrete sensing areas with a sample fluid flow. Immobilization of a ligand upon the discrete sensing area, followed by selective contact with an analyte contained within the sample fluid flow, allows analysis by a wide variety of techniques. Sensitized sensing surfaces, and sensor devices and systems are also provided.

Methods for capturing analytes associated with surface-bound ligands are disclosed. The methods involve eluting analytes from surface-bound ligands with a first liquid to generate free analytes, and capturing the free analytes with a solid capturing material within the first liquid to generate a first liquid containing captured analytes. The first liquid may be a flowing liquid or a non-flowing liquid, and the surface to which the surface-bound ligand is attached may be a sensing surface, such as a biosensor, or a non-sensing surface. The captured analytes may be further consolidated at a location removed from the surface-bound ligand, eluted from the solid capturing material with a second liquid, and used for subsequent analysis or procedures.

Method for capturing analytes eluted from surface-bound ligands

Multivalent and multispecific binding proteins and their use

Surface regeneration of affinity biosensors and characterization of biomolecules associated therewith by multivariate technique employing cocktails of regeneration agents to optimize regeneration of biosensor surface and/or characterize biomolecules associated therewith. Kits and stock solutions for use in the context of this invention, as well as associated computer algorithms are also disclosed.

Surface regeneration of biosensors and characterization of biomolecules associated therewith

Surface-sensitive detection of hybridization at equilibrium

Disclosed is apparatus for detecting the presence of an analyte of interest in a sample, comprising an immobilised binding partner comprising a solid support and a reversibly binding receptor which is capable of binding to the analyte of interest thereby causing a measurable change in a property of the immobilised partner, and detection means for detecting the measurable change and a method for detecting the presence of an analyte of interest.

Analyte detection

Growing community of inventors

Uppsala, Sweden

Magnus Malmqvist