Agilent Technologies, Inc.

Magdalena Anna Ostrowski

Arthur Schleifer

Kevin P Killeen

Jian Bai

Karla M Robotti

Brian Phillip Smart

Viorica Lopez-Avila

Jean L Truche

Rudolf Grimm

Gregory Staples

Microfluidic devices and methods for analyzing glycan profiles of glycoproteins are provided. Some embodiments of the devices comprise a deglycosylation column for cleaving glycans, an optional cleaning column for removing proteins, a trapping column for enriching glycans, and a separation column for resolving glycans. The devices and methods significantly improve the speed and sensitivity of glycan analysis.

Microfluidic glycan analysis

A microfluidic device for glycan analysis includes a deglycosylation column comprising a glycosidase attached to a solid support; a tagging column comprising a reactive ester for reaction with an amino group, wherein the tagging column is arranged downstream of the deglycosylation column; an analytical column comprising a stationary phase capable of separating a derivatized glycan; and a plurality of inlet/outlet ports configured to connect with channels on a switching element to form flow paths.

Derivatization of PNGase F released glycans on an HPLC chip

MALDI sample holders and methods of using and making the same are provided. The MALDI sample holders are configured for use in matrix assisted laser desorption/ionization and include a planar substrate having a surface and at least one fluid retaining structure present on the surface. The fluid retaining structure includes a material that changes from a first fluid state to a second solid state in response to a stimulus. Also provided are methods of using the subject MALDI sample holders in a matrix-assisted laser desorption/ionization protocol, as well as methods of producing the subject MALDI sample holders. Kits for use in practicing the subject methods are also provided.

Matrix-assisted laser desorption/ionization sample holders and methods of using the same

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Mountain View, CA, United States of America

Magdalena Anna Ostrowski