Centre National De La Recherche Scientifique

Institut National De La Recherche Agronomique

Institut National Des Sciences Appliquees De Toulouse

Institut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement

Universitë De Bordeaux

Institut Polytechnique De Bordeaux

Adisseo France Sas

Institut National Des Sciences Appliquees Toulouse

Institut Pasteur

Université Paul Sabatier (Toulouse III)

Centre National De La Recherche Scientifique-Cnrs

Institute National Des Sciences Appliquees De Toulouse

Institut National Des Sciences Appiquees De Toulouse (Insat)

Tate & Lyle Ingredients France Sas

Pierre Monsan

Magali Remaud-Simeon

Claire Moulis

Isabelle Andre

Gabrielle Potocki-Veronese

Marlene Vuillemin

Sandrine Morel

Robert Huet

Thomas Walther

Christopher Topham

Jean-Marie Francois

Marlène Vuillemin

Marion Claverie

Helene Cordier

Denis Pompon

Pierre F Monsan

Michel Lopez

Laurence Mulard

Stéphane Grelier

Philippe Valet

Sophie Barbe

Etienne Severac

Thierry Naeye

Alexandra Einerhand

Susan M Potter

Frédéric Peruch

Philippe Urban

Gilles Joucla

Remy Burcelin

Jean-Sebastien Saulnier-Blache

Akli Ben Imeddourene

Mounir Benkoulouche

Louis-Antoine Barel

Zhaohu Hu

Guillaume Le Heiget

Florent Grimaud

Patrick Lucas

Marguerite Dols-Lafargue

Catherine Garnier

Agnès Sabate

Catherine Fontagne-Faucher

Yannick Malbert

Emmanuelle Cambon

Yoan Brison

Alvaro Lafraya

David Guieysse

Pierre Frederic Monsan

Cordier Hélène

The present invention provides protected tetrasaccharides, their process of preparation and their use in the synthesis of oligosaccharides, in particular fragments of O-antigens from, for example of serotype 1a, 1b, 2a, 2b, 3a, X, 4a, 4b, 5a, 5b, 7a or 7b.

Protected tetrasaccharides, their process of preparation and their use as transglucosylase acceptor substrates in the chemo-enzymatic synthesis ofspecific oligosaccharides

Polypeptides having the ability to specifically form connections of glucosyl units in alpha 1,3 on an acceptor having at least one hydroxyl moiety are presented. The polypeptides include i) the pattern I of sequence SEQ ID NO: 1, ii) the pattern II of sequence SEQ ID NO: 2, iii) the pattern III of sequence SEQ ID NO: 3, and iv) the pattern IV of sequence SEQ ID NO: 4, or derivates from one or several of said patterns, wherein the polypeptide furthermore has an aspartic residue (D) at position 5 of the pattern II (SEQ ID NO: 2), a glutamic acid residue (E) at position 6 of the pattern III (SEQ ID NO: 3) an an aspartic acid residue (D) at position 6 of the pattern IV (SEQ ID NO: 4). Methods for producing acceptors connected to glucosyl units in alpha 1,3 using the polypeptides are also provided.

Polypeptide having the ability to form connections of glucosyl units in alpha-1,3 on an acceptor

Polypeptides having the ability to specifically form connections of glucosyl units in alpha 1,3 on an acceptor having at least one hydroxyl moiety are presented. The polypeptides include i) the pattern I of sequence SEQ ID NO: 1, ii) the pattern II of sequence SEQ ID NO: 2, iii) the pattern III of sequence SEQ ID NO: 3, and iv) the pattern IV of sequence SEQ ID NO: 4, or derivates from one or several of said patterns, wherein the polypeptide furthermore has an aspartic residue (D) at position 5 of the pattern II (SEQ ID NO: 2), a glutamic acid residue (E) at position 6 of the pattern III (SEQ ID NO: 3) and an aspartic acid residue (D) at position 6 of the pattern IV (SEQ ID NO: 4). Methods for producing acceptors connected to glucosyl units in alpha 1,3 using the polypeptides are also provided.

Polypeptide having the capacity to form alpha-1,3 glucosyl unit branchings on an acceptor

The present invention relates to a method for producing a glycosylated synthon or a monomer. Said method includes at least one step of placing at least one glycan-saccharase in the presence of at least one hydroxylated synthon and at least one saccharose. The invention also relates to a method for producing a glyco(co)polymer, including polymerizing at least two monomers separately obtained from the enzymatic glycosylation method according to the invention, and to a method for producing a glyco(co)polymer, preferably a block glyco(co)polymer, including coupling at least two monomers separately obtained from the enzymatic glycosylation method according to the invention.

Enzymatic production of glycosylated synthons

The invention relates to a method for producing derivatives of O-&#x3b1;-glucosylated flavonoid, comprising at least one step of incubating a glucansucrase with a flavonoid and at least one sucrose, the flavonoid being a flavonoid which is monohydroxylated or hydroxylated in a non-vicinal manner on the B cycle. The invention also relates to novel O-&#x3b1;-glucosylated flavonoid derivatives, and to the use thereof. 

Flavonoids O-A-glucosylated on the B cycle, method for the production thereof and uses

A method of producing 2,4-dihydroxybutyric acid (2,4-DHB) by a synthetic pathway that includes transforming malate into 4-phospho-malate using a malate kinase, then transforming 4-phospho-malate into malate-4-semialdehyde using a malate semialdehyde dehydrogenase, and then transforming malate-4-semialdehyde into 2,4-DHB using a DHB dehydrogenase.

Method of production of 2,4-dihydroxybutyric acid

The invention relates to: &#x2014;a protein having dextran-saccharase activity and the sequence SEQ ID NO: 1 for an amino acid sequence; &#x2014;a protein having dextran-saccharase activity and the sequence SEQ ID NO: 2 for an amino acid sequence; &#x2014;a complex containing a substrate and a protein with dextran-saccharase activity &#x2014;a method for synthesizing dextrans; and &#x2014;dextrans. The invention also relates to a method for synthesizing gluco-oligosaccharides and gluco-conjugates and to the resulting products.

Protein with dextran-saccharase activity, and uses

The subject matter of the invention is dextrans which have between 95% and 99% of &#x3b1;-1,6 glucosidic bonds, a weight-average molar mass Mat least equal to 0.7&#xd7;10g.mol, and a dispersity index D of between 1.3 and 3. The invention also relates to a dextran saccharase which makes it possible to produce such dextrans, and to a method for producing said dextrans.

Very high molar mass dextrans

The present invention relates to a recombinant process for the production of truncated or mutated dextransucrases while conserving the enzymatic activity or their specificity in the synthesis of the &#x3b1;-1,6 bonds. The present invention relates to nucleic acid sequences of truncated or mutated dextransucrases, vectors containing the nucleic acid sequences and host cells transformed by sequences encoding truncated or mutated dextransucrases. In another aspect, the invention concerns a method for producing, in a recombinant manner, truncated or mutated dextransucrases which conserve their enzymatic activity or which conserve their specificity in the synthesis of &#x3b1;-1,6 bonds and can produce, from saccharose, dextrans with high molar mass and modified rheological properties compared with the properties of dextran obtained with the native enzyme and isomalto-oligosaccharides with a controlled molar mass and dextrans. The dextrans and isomalto-oligosaccharides of the invention can be used namely as texturing agents or as prebiotics.

Construction of new variants of dextransucrase DSR-S by genetic engineering

A method of producing 2,4-dihydroxybutyric acid (2,4-DHB) by a synthetic pathway that includes the transforming malate in 4-phospho-malate using a malate kinase, said 4-phospho-malate being transformed in malate-4-semialdehyde using a malate semialdehyde dehydrogenase and said malate-4-semialdehyde being transformed in 2,4-DHB using a DHB dehydrogenase.

The invention relates to the use of prebiotics for the preparation of food or pharmaceutical compositions which are intended for the treatment and/or prevention of hyperglycaemic syndromes and, in particular, for the treatment of Type II diabetes and/or the prevention of the onset of Type II diabetes in patients with a predisposition to developing the type of diabetes. The invention also relates to the food and pharmaceutical compositions containing the prebiotics.

Use of prebiotics, preferably glucooligosaccharide, for the prevention of the onset of type II diabetes

Growing community of inventors

Ramonville, France

Magali Remaud-Simeon