Centre National De La Recherche Scientifique

Institut Curie

Societe De Developpement Et De Recherche Industrielle

Elvesys

Bforcure

Urgo Recherche Innovation Et Developpement

Maël Le Berre

Yanjun Liu

Adrien Plecis

Matthieu Piel

Yong Chen

Walter Minnella

Mathieu Velve Casquillas

A microfluidic sample chip to test biological samples, especially for a PCR-type and/or fluorescence assay. The chip being in the shape of a hollow block having at least one chamber delimited by an upper wall, a lower wall and at least one side wall, into which a sample can be introduced for testing. The lower wall of the block is made of a material with a high thermal conductivity and the upper wall is made of a material with a low thermal conductivity. Preferably, the upper wall is preferably permeable to radiation in the visible spectrum between 400 and 700 nm. The block having at least two openings through which the sample can be introduced into at least one of the chambers and through which the air present in the chamber can be evacuated when the sample is introduced.

Microfluidic sample chip, assay system using such a chip, and PCR method for detecting DNA sequences

A microfluidic thermalization chip, a system using such a chip and a PCR method for detecting DNA sequences. The chip contains a block of material in which a cavity is configured to contain at least one fluid. The cavity includes at least one inlet orifice and at least one outlet orifice. The inlet orifice for the fluid is connected to at least one, preferably at least two, fluid-injecting channels. Further, the chip includes at least one microfluidic channel for bypassing the cavity. The channel is connected by a first end to at least one of the fluid-injecting channels. The junction between the bypassing channel and the fluid-injecting channel is located at a distance L from the inlet orifice of the fluid-injecting channel. The distance L is preferably smaller than 2 cm.

Microfluidic thermalization chip with variable temperature cycles, system using such a chip and PCR method for detecting DNA sequences

A system to measure the flow rate of a liquid in a microfluidic circuit. The system includes a vessel that is partially filled with the liquid, a gaseous ceiling above the vessel and a pressure regulator to maintain the pressure of the gas in the gaseous ceiling at a predetermined value P. A capillary pipe to extract the liquid from the vessel and to output the liquid at a pressure Plower than P. A first inlet of the pressure sensor is connected to the gaseous ceiling, a second inlet of the pressure sensor is connected to the capillary pipe, and the outlet of the pressure sensor outputs a signal as a function of the pressure difference (P&#x2212;P), which is a measurement representing the flow rate of pressurized Pliquid supplied to the microfluidic circuit.

System for measuring flow of a liquid in a microfluidic circuit by determining gas and liquid pressures

A micro-structured substrate to support a liquid sample having a lower face and an upper face. At least one surface cavity of volume Vopening onto the upper face and forming a zone for analyzing the liquid sample. A first groove having a first volume V, positioned around each surface cavity and opening onto the upper face thereof. The substrate also includes at least one second groove opening onto the upper face thereof and positioned around the first groove. The sum of the volumes of the second grooves is equal to V. The volume V+Vis greater than or equal to 0.05 V, preferably 0.1 V. An analysis assembly including the substrate and the use thereof is also contemplated herein.

Substrate for supporting liquid sample, an assembly comprising such a substrate and use thereof

The subject matter of the present invention is a device for guiding cell migration comprising a substrate having a textured surface intended to be brought into contact with cells, said textured surface having an anisotropic three-dimensional structure consisting of a network of projections inclined relative to the normal to the plane formed by said textured structure, in the direction imparted by said anisotropic structure. The invention also concerns, according to another aspect, a method for guiding cell migration including the bringing into contact of cells with a substrate having a textured surface and an anisotropic three-dimensional structure, said structure consisting of projections inclined as previously described. The device or method according to the invention can in particular be applied in the fields of dermatology, implantology and tissue engineering.

Device for guiding cell migration and guiding method implementing such a device

A guiding device in which cells are confined between a support surface and a textured surface of a substrate, said textured surface having an anisotropic three-dimensional structure having a repeating pattern that repeats according to a repeat axis, said repeating pattern having a succession of guide spaces adjacent to one another according to the repeat axis, each of said guide spaces being capable of receiving at least one portion of one of the cells and being oriented according to a direction of anisotropy to guide a movement of the cells in the direction of anisotropy.

Growing community of inventors

Paris, France

Maël Le Berre