Institut National De La Sante Et De La Recherche Medicale

Institut Pasteur

Madeleine Cochet

Philippe Kourilsky

Christophe Pannetier

Sylvie Darche

The present invention relates to a process for determining the quantity of a DNA fragment of interest in a sample wherein:

Process for determining the quantity of a DNA fragment of interest by a

In a method for describing repertoires of antibodies (Ab) and T cell recers (TcR) of the immune system of an individual, reverse transcription is carried out on the mRNA contained in a biological sample, separate amplifications are then carried out on the transcription product (or directly on the DNA extracted from the sample) by a PCR type method for each primer pair V, C, where V corresponds to a variable segment of the repertoire of interest and C hybridizes on the constant segment of the repertoire of interest. For each J segment of the labelled repertoire, an elongation step is carried out on each of the amplification products using a specific oligonucleotide of this segment J as primer and the amplification product as matrix. For each elongation product corresponding to a triplet (V, C)J thus obtained, the size of the different elongation products is revealed. The description of the repertoires corresponds to a VCJ triplet and to the element size for each element of the repertoire.

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Fontenay-aux-Roses, France

Madeleine Cochet