Koninklijke Philips Corporation N.V.

Scienion Ag

Maatje Koets

Menno Willem Jose Prins

Toon Hendrik Evers

Albert Hendrik Jan Immink

Marius Iosif Boamfa

Joannes Baptist Adrianus Dionisius Van Zon

Thea Van Der Wijk

Menno W J Prins

Remco Den Dulk

Joost Hubert Maas

Dirkjan Bernhard Van Dam

Aart Van Amerongen

Albert H J Immink

Michel J M Bruyninckx

Hugo Antonie Arends

Jeroen Veen

A device detects a target molecule in a sample in a sample container to measure the target molecule. A first particle is functionalized with a first binding molecule capable of specifically binding to said target molecule. A surface structure includes a second binding molecule. The surface structure covers a flat sensor or is present on a second particle. The first particle is capable of binding the second binding molecule of the surface structure directly or indirectly. The first and/or second binding molecule is indirectly attached to the particle surface of the first and/or second particle and/or the flat sensor surface via a long and rigid linker molecule. A length and a consistency of the linker molecule is selected such as to result in an average extension length of the linker of more than 60 nm. A number of particle clusters or of bound particles is directly or inversely related to an amount of the target molecules present in the sample.

Long rigid spacers to enhance binding kinetics in immunoassays

The invention relates to a substance determining apparatus and method for determining a substance within a fluid. Particles attach to the substance and bind to a binding surface (), wherein a particle release curve being indicative of a release of bound particles from the binding surface () is determined, and a part of the particle release curve caused by particles bound to the binding surface via a predefined kind of binding is determined based on the temporal behaviour of the particle release curve. The substance within the fluid is determined based on the part of the particle release curve. The substance can therefore be determined based on particles which are bound to the binding surface via a certain kind of binding, i.e. other kinds of binding substantially do not affect the determination of the substance, thereby improving the accuracy of determining the substance.

System, apparatus and method for determining a substance within a fluid

The present invention discloses microfluidic devices with a valve-like structure (3), through which magnetic particles can be transported with minimal transport of fluids. This allows sequential processing of the magnetic particles.

Multi-compartment device with magnetic particles

A method of amplifying nucleic acids and determining the amount of amplified nucleic acids uses magnetic detection. The detection can be performed during the amplification process of the nucleic acid. During the detection, the amplified nucleic acid is bound to a sensor via a biological molecule.

Amplification of nucleic acids with magnetic detection

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Eindhoven, Netherlands

Maatje Koets