Koninklijke Philips Corporation N.v.

Maarten M J W Van Herpen

Derk J W Klunder

Hendrik Roelof Stapert

Sjoerd Stallinga

Bernardus H W Hendriks

Erik M H P Van Dijk

Dirk L J Vossen

Henk Van Houten

A luminescence sensor, comprising a non-transparent substrate structure () having at least one aperture () intended to comprise an analyte and a transparent substrate structure (), which is arranged to or adjacent said first structure (). The aperture has a smallest lateral dimension, which is smaller than half the effective wavelength of an excitation radiation, such as light at a wavelength of 700 nm, resulting in an effective wavelength in water of about 538 nm. The transparent structure has a trench () with a surface portion provided with ligands with an affinity towards a target molecule. The trench results in that a luminophore attached to the target molecule will be positioned at the entrance surface of the aperture, where the excitation energy is largest.

Aperture biosensor with trenches

A device for monitoring radiation emitted by luminophores present in an analyte fluid of a wiregrid biosensor. The monitoring device comprises a non-polarized light source () for illuminating the wiregrid biosensor for exciting fluorescent labels arranged in the analyte fluid of said biosensor. A detector () detects radiation emitted by the labels after excitation. A polarizing filter () is arranged in between the transparent substrate and the detector for suppression of background emission radiation from labels positioned outside apertures in the wiregrid, in the analyte fluid.

Wiregrid monitor device

A method of determining a color transformation for images of biological material includes preparing a first set of biological test objects using a first preparation method, and preparing a second set of biological test objects using a second preparation method. Each test object in the second set of test objects corresponding to a counterpart test object in the first set of test objects, the test object and its counterpart being of the same biological type of material. For each test object in the first and second set of test objects, the color of the test object is determined thereby generating a first and second set of colors The method further includes generating a conversion table indicating a mapping between the colors in the first set of colors and the corresponding colors in the second set of colors. The first and second preparation methods include first and second staining methods, respectively.

Colour management for biological samples

There is provided a method of detecting a presence of a luminophore in a detection volume comprising providing excitation radiation in said detection volume. A luminophore is provided in said detection volume being excitable by said excitation radiation. The luminescent radiation is detected to identify the presence of said luminophore in said detection volume. In one aspect of the invention, said luminophore is selected to emit luminescent radiation having a wavelength in said medium that is larger than twice said smallest dimension; and wherein said luminophore is selected to be excitable by excitation radiation having a wavelength in said medium that is smaller than twice said smallest dimension. Accordingly, luminescent radiation is blocked from entering the detector but for the portion present on an interface of the aperture.

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Eindhoven, Netherlands

Maarten M J W Van Herpen