Syngenta Mogen B.v.

Zeneca Mogen B.v.

Maarten Hendrik Stuiver

Lambertus Henricus Simons

Anne Silene Ponstein

Jer&ocirc;me Hubertina Henricus Victor Custers

Leo Sjoerd Melchers

Marianne Beatrix Sela-Buurlage

Stephan Andreas Ohl

Johanna Pieternella Els Van Deventer-Troost

Floor Hendrik Sijbolts

Bernardus Martinus Maria Dekker

Jerome Hubertus Henricus Victor Custers

Sietske Hoekstra

Wessel Lageweg

Hendrik Tigelaar

Oscar Johanna Maria Goddijn

Nicolas Elzinga

The invention provides novel Ti-plasmid vectors for-medicated plant cell transformation. Plamsids of the invention are constructed with a DNA sequence (ie., a negative selectable maker or a DNA sequence that inhibits DNA unwinding) flanking the T-DNA bordered gene of interest that reduces or eliminates the occurrence of transformants with vector read-through DNA sequence. The invention also provides methods of plant cell transformation utilizing these novel vectors.

Plasmids for plant transformation and method for using the same

The invention describes new promoters built from elements from a set of promoters which have a complementary expression pattern.

Constitutive plant promoters

The present invention provides an isolated protein obtainable from a plant source which has antifungal activity, specifically anti-Phytophthora activity and/or anti-Pythium activity and a molecular weight of about 55-65 kDa as judged by SDS PAGE-electrophoresis, an isolated DNA sequence comprising an open reading frame capable of encoding a protein according to the invention, preferably characterized in that it comprises an open reading frame which is capable of encoding a protein depicted in SEQ ID NO. 16, SEQ ID NO. 57, SEQ ID NO. 70, SEQ ID NO. 72 or SEQ ID NO. 74 or muteins thereof, and DNA capable of hybridizing therewith under stringent conditions. The invention further comprises plants incorporating chimeric DNA capable of encoding a protein according to the invention, and wherein the protein is expressed. Also shown is the carbohydrate and preferably hexose oxidating activity of said protein. Also methods are provided for combating fungi, especially Phytophthora and Pythium species, using a protein or a host cell capable of producing the protein.

Antifungal proteins, DNA coding therefore, and hosts incorporating same

The invention comprises a method for the induction of pathogen resistance in plants characterized by transforming a plant with a polynucleotide sequence comprising a pathogen inducible promoter which regulates the expression of a plant signal transduction protein or a homologue thereof which when constitutively expressed gives rise to a hypersensitive response in plants. Alternatively, the invention comprises a method for the induction of pathogen resistance in plants characterized by transforming a plant with a polynucleotide sequence comprising a pathogen inducible promoter which regulates the expression of a compound which is able to alleviate the inhibitory effect of a protein on the signal transduction pathway leading to a hypersensitive response in plants.

Method for the induction of pathogen resistance in plants

This invention describes pathogen-inducible promoters which normally drive expression of plant hexose oxidases, especially those which can be isolated from and , more specifically those promoters which naturally are the regulatory regions driving expression of the hexose oxidase MS59 and WL64, respectively. Also claimed are chimeric constructs where these pathogen-inducible promoters drive expression of antipathogenic proteins or of proteins which can elicit a hypersensitive response.

Pathogen-inducible promoter

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Oegstgeest, Netherlands

Maarten Hendrik Stuiver