Corning Incorporated

Ma Sha

Jinlin Peng

Bernice I Feuer

Kathy Marie Youngbear

Methods and compositions useful for capturing proteins or peptides from a sample mixture and analyzing the captured proteins or peptides via high throughput electrospray ionization (ESI) or matrix-assisted laser desorption/ionization mass spectrometry (HT MALDI MS) are provided. The methods and compositions are useful in large scale, simultaneous analyses of proteins and peptides present in a cell(s), tissue(s), or biological fluid(s).

Peptide or protein-capturing surfaces for high throughput MALDI mass spectrometry

The present invention includes a microplate for performing crystallography studies. In particular, the microplate has a frame that includes a plurality of wells formed therein. Each well includes a first well and a second well. The first well includes a relatively large reservoir capable of receiving a reagent solution. The second well includes a relatively small reservoir having a substantially concaved form capable of receiving a protein solution and a reagent solution. The second well is suspended above the first well such that space on the plate is conserved and to enable protein crystal growth utilizing a hanging drop vapor diffusion crystallization process.

Protein crystallography hanging drop multiwell plate

The present invention includes a microplate for performing crystallography studies and methods for making and using such microplates. In particular, the microplate has a frame which includes a plurality of wells formed therein. Each well includes a first well and a second well. The first well includes a relatively small reservoir having a substantially concaved form capable of receiving a protein solution and a reagent solution. The second well includes a relatively large reservoir capable of receiving a reagent solution that has a higher concentration than the reagent solution within the first well, wherein the protein solution and the reagent solution within the first well interact with the reagent solution within the second well via a vapor diffusion process which enables the formation of protein crystals within the first well. The microplate may be sized so that it can be handled by a robotic handling system and/or a liquid handling system.

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Cambridge, MA, United States of America

Ma Sha