Becton Dickinson and Company Limited

M Scott Ulrich

Michael L Marshall

Melody M H Kuroda

Michael Justin Lizzi

Dwight Livingston

Mark A Talmer

Thomas D Haubert

James A Prescott

Gregory S Kramer

Adam C Curry

Randy J Somerville

Scott Nicholas Danhof

Artis R Lockhart

Jeffrey S Krause

Ernie Ritchey

Ammon David Lentz

Robert L Campbell

Kristin Weidemaier

Andrea Liebmann-Vinson

Eric Fallows

David S Sebba

Kevin G Dolan

Donald E Gorelick

Miroslaw Bartkowiak

Wendy Dale Woodley

Spencer Lovette

Erin Gooch Carruthers

Jeffrey S Ojala

Eric A Fallows

Melody MH Kuroda

Vessel assembly

Provided herein are methods, systems, and devices for detecting and/or identifying one or more specific microorganisms in a culture sample. Indicator particles, such as surface enhanced Raman spectroscopy (SERS)-active nanoparticles, each having associated therewith one or more specific binding members having an affinity for the one or more microorganisms of interest, can form a complex with specific microorganisms in the culture sample. Further, agitating magnetic capture particles also having associated therewith one or more specific binding members having an affinity for the one or more microorganisms of interest can be used to capture the microorganism-indicator particle complex and concentrate the complex in a localized area of an assay vessel for subsequent detection and identification. The complex can be dispersed, pelleted, and redispersed so that the culture sample can be retested a number of times during incubation so as to allow for real-time monitoring of the culture sample.

Methods, systems, and devices for detecting and identifying microorganisms in microbiological culture samples

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Columbus, OH, United States of America

M Scott Ulrich