American Cyanamid Company

Molecular Genetics Research and Development Limited Partnership

Lynn W Enquist

Roger J Watson

John H Weis

Henry J George

John W Weis

Richard A Krzyzek

Methods and compositions are provided for the cloning and expression of Herpes Simplex Virus (HSV type 1 or type 2) glycoprotein (gD) gene in single-cell host organisms. Also described are methods for culturing these novel single-cell organisms to produce HSV-gene products. The HSV gD-related polypeptides produced by the recombinant DNA techniques described herein may be formulated for use as immunogens in vaccines to protect against HSV-1 and HSV-2 infections.

Production of herpes simplex viral porteins

Methods and composition are provided for the cloning and expression of a Herpes Simplex Virus (HSV) glycoprotein (gD) gene in single-cell host organisms. Also described are methods for culturing these novel single-cell organisms to produce the HSV-gene product. The HSV gD-related polypeptide produced by the recombinant DNA techniques described herein may be formulated for use as an immunogen in a vaccine to protect against HSV-1 and HSV-2 infection.

Production of herpes simplex viral protein

Methods are provided for stabilization and purification of proteins produced in host cell systems. The methods provide for significant stabilization of any unfused non-bacterial protein that is produced in the same host cell that is producing an aggregate-forming fusion protein or any aggregate-forming protein.

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Excelsior, MN, United States of America

Lynn W Enquist