Biomerieux, Inc.

Biomerieux Sa

Idg (UK) Limited

Lyle Armstrong

Arthur James

Sylvain Orenga

Daniel Monget

An alizarin-based chromogenic substrate, a composition containing the substrate, and its use to detect the presence of an enzymatic activity are disclosed. The substrate is particularly applicable in the field of biological diagnosis.

Alizarin-based chromogenic substrates, their uses and composition containing same

The invention concerns novel enzymatic substrates of general formula (I) wherein: X represents a nitrogen atom or a carbon atom substituted with a phenyl group, said phenyl group being optionally substituted in meta or para position: Y and Z represent a hydrogen atom when X represents a carbon atom or Y and Z represent together an ether, thioether or amine bond optionally substituted with an alkyl or aryl group; Rand Rindependently represent each H, Cl, F, I, Br and can be identical or different or Rand Rtogether represent a substituted or non-substituted fused benzene ring, Rand Rindependently represent each H, Cl, F, I, Br and can be identical or different; R represents a group such that the O&#x2014;R bond is capable of being hydrolysed by an enzyme. The invention also concerns a method for synthesizing said substrates, a composition, a kit and a method for detecting at least one micro-organism using said enzymatic substrate.

Enzymatic substrate, synthesis method and uses

Method and agent for determining a deaminase enzymatic activity

The invention concerns a chromogenous substrate for detecting the presence of at least one enzyme enzymatic activity. The invention also concerns a method for using such a substrate. The invention further concerns a method for identification based on such substrates and a device for implementing said identification method. The invention is characterized in that the substrate consists of at lease two molecules, a first molecule consisting of a non-chromogenous marker part associated with at least a specific target part for the enzyme and a second molecule consisting of a non-chromogenous part, and the non-chromogenous marker part, once it is released, reacts with the second molecule to form a chromogenous molecule. The invention is particularly applicable in bacteriology.

Chromogenic substrates for detecting bacterial hydrolases

The invention concerns novel 6- or 7-substituted derivatives of esculetin (6,7-dihydroxy-2H-1-benzopyran-2-one), their synthesis, and their application as substrates for the detection of micro-organisms in samples where a derivative is enzymatically cleaved to release a colored or fluorogenic marker which has a low tendency to diffuse through agar or other aqueous environments.

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Ashington, United Kingdom

Lyle Armstrong