US Government As Represented by the Secretary of the Army

Wisconsin Alumni Research Foundation

Luther E Lindler

Richard Lloyd Warren

Frederick R Blattner

Gerhardt G Schurig

Stephen M Boyle

Nicole T Perna

Valerie Burland

Mikeljon Nikolich

Susan C Straley

David Hoover

Nammalwar Sriranganathan

Robert D Perry

George F Mayhew

Gregory V Plano

Debra J Rose

Jacqueline D Fetherston

Ted L Hadfield

John R McOulston

The complete DNA sequence of three plasmids from the bacterium , the causative agent for bubonic plague, have been determined and are set forth. The open reading frames, or protein coding regions, of the plasmids have been determined. The DNA sequence and ORF information is useful for the creation of diagnostic, prophylactic and therapeutic tools for combating the disease caused by this agent.

Plasmid DNA from Yersinia pestis

Live Brucella vaccines and methods for preparing the live vaccines protective against brucellosis are described. The vaccines are prepared by introducing a deletion in the rfbU gene of a strain of Brucella which results in attenuation of the strain while retaining the desired immunogenicity to initiate a protective immunogenic response. Other strains with varying levels of attenuation are described.

Live vaccine against Brucellosis

Growing community of inventors

Wheaton, MD, United States of America

Luther E Lindler