University of Utah Research Foundation

Biofire Defense, LLC

Idaho Technology, Inc.

Her Majesty the Queen in Right of Canada As Represented by the Dept. of

Biofire Diagnostics, LLC

Utah Valley University

Luming Zhou

Carl Thomas Wittwer

Virginie E Dujols

Gudrun Reed

Philip Seth Bernard

Mark Aaron Poritz

Robert Andrew Palais

Leonard Brent Selinger

Cecil Wallace Forsberg

Hee Dong Bae

Kuo Joan Cheng

Lindsey Jay Yanke

Joseph A Holden

Carlynn Willmore-Payne

Cameron N Gundry

Robert Palais

Jason T Mckinney

Jospeh Holden

Virginie Dujols

Methods are provided for nucleic acid analysis wherein a target nucleic acid is mixed with a dsDNA binding dye to form a mixture. Optionally, an unlabeled probe is included in the mixture. A melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.

Nucleic acid melting analysis with saturation dyes

Methods and kits for performing polymerase chain reaction (PCR) and melting analyses to determine copy number variation and/or gene expression are disclosed herein. Related uses of such methods and analyses are also disclosed herein.

Methods of detecting copy number variation

Methods are provided for nucleic acid analysis. In an illustrative method, allele amplification bias is used to amplify preferentially a target nucleic acid that is present in a low allele fraction.

Allele amplification bias

Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.

Amplicon melting analysis with saturation dyes

Methods and kits are provided for nucleic acid analysis. In an illustrative method a target nucleic acid is amplified using a first primer and a second primer, wherein the first primer comprises a probe element specific for a locus of the target nucleic acid and a template-specific primer region, and the probe element is 5' of the template-specific primer region, subsequently allowing the probe element to hybridize to the locus to form a hairpin, generating a melting curve for the probe element by measuring fluorescence from a dsDNA binding dye as the mixture is heated, wherein the dye is not covalently bound to the first primer, and analyzing the shape of the melting curve. Kits may include one or more of the first and second primers, the dsDNA binding dye, a polymerase, and dNTPs.

Primers for melting analysis

Methods and kits are provided for nucleic acid analysis. In an illustrative method, Snapback-ARMS primers are used to amplify preferentially a target nucleic acid that is present in a low allele fraction. In another embodiment, tailed primers are used to identify the preferentially amplified allele.

Methods, compositions, and kits for rare allele detection

Methods are provided for nucleic acid analysis. In an illustrative method, Snapback-ARMS primers are used to amplify preferentially a target nucleic acid that is present in a low allele fraction. In another embodiment, tailed primers are used to identify the preferentially amplified allele.

Novel phytases derived from ruminal microorganisms are provided. The phytases are capable of catalyzing the release of inorganic phosphorus from phytic acid. Preferred sources of phytases include Selenomonas, Prevotella, Treponema and Megasphaera. A purified and isolated DNA encoding a phytase of Selenomonas ruminantium JY35 (ATCC 55785) is provided. Recombinant expression vectors containing DNA's encoding the novel phytases and host cells transformed with DNA's encoding the novel phytases are also provided. The novel phytases are useful in a wide range of applications involving the dephosphorylation of phytate, including, among other things, use in animal feed supplements.

DNA sequences encoding phytases of ruminal microorganisms

Growing community of inventors

Salt Lake City, UT, United States of America

Luming Zhou